Biodiversitas Journal of Biological Diversity
Vol. 20 No. 7 (2019)

The potential of yeasts from the oil-contaminated soil and mangrove rhizosphere for degrading crude oil

NUR HIDAYATUL ALAMI (Department of Biology, Faculty of Mathematics and Natural Sciences, Institut Teknologi Sepuluh Nopember. Jl. Raya ITS, Keputih, Sukolilo, Surabaya 60111, East Java, Indonesia)
LUTHFI LAILATUL MAULIDIYA (Department of Biology, Faculty of Mathematics and Natural Sciences, Institut Teknologi Sepuluh Nopember. Jl. Raya ITS, Keputih, Sukolilo, Surabaya 60111, East Java, Indonesia)
N D KUSWYTASARI (Department of Biology, Faculty of Mathematics and Natural Sciences, Institut Teknologi Sepuluh Nopember. Jl. Raya ITS, Keputih, Sukolilo, Surabaya 60111, East Java, Indonesia)
ENNY ZULAIKA (Department of Biology, Faculty of Mathematics and Natural Sciences, Institut Teknologi Sepuluh Nopember. Jl. Raya ITS, Keputih, Sukolilo, Surabaya 60111, East Java, Indonesia)
MAYA SHOVITRI (Department of Biology, Faculty of Mathematics and Natural Sciences, Institut Teknologi Sepuluh Nopember. Jl. Raya ITS, Keputih, Sukolilo, Surabaya 60111, East Java, Indonesia)



Article Info

Publish Date
12 Jun 2019

Abstract

Abstract. Alami NH, Maulidiya LL, Kuswytasari ND, Zulaika E, Shovitri M. 2019. The potential of yeasts from the oil-contaminated soil and mangrove rhizosphere for degrading crude oil. Biodiversitas 20: 1833-1838. Bioremediation of crude oil contaminated soil eventually depends on the activities of microorganisms to degrade contaminants. Bioaugmentation, through the addition of microbial culture to contaminated soil, can be used to enhance degradation rates. This research aimed to determine the effect of yeast formulations isolated from the oil-contaminated soil and mangrove rhizosphere to degrade crude oil and evaluate the most effective yeast formulation and incubation timefor crude oil degradation. This research was conducted by subculturing yeast in the slant agar medium, screening hydrocarbonoclastic yeast in crude oil- Bushnell Hass Mineral Salt (BHMS) medium, biodegradation test, measuring the total cell number with Total Plate Count (TPC), Total Petroleum Hydrocarbon (TPH) test with the gravimetric method, and measuring soil moisture content. The design of the research was Completely Randomized Factorial Design with 3 replications. Data were analyzed quantitatively by using statistical tests for TPC and TPH, while a descriptive qualitative test analyzed soil moisture. The result showed that yeast formulation and incubation time affected crude oil degradation. An effective yeast consortium for oil degradation is T20 consortium with an incubation period of 30 days, which result in 72.26 % oil degradation.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...