Biodiversitas Journal of Biological Diversity
Vol. 20 No. 6 (2019)

Isolation and partial characterization of alginate extracted from Sargassum polycystum collected from three habitats in Banten, Indonesia

NIKEN DHARMAYANTI (Program of Conservation Biology, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia. Jl. Lingkar UI, Depok 16242, West Java, Indonesia)
JATNA SUPRIATNA (Program of Conservation Biology, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia. Jl. Lingkar UI, Depok 16242, West Java, Indonesia)
ABINAWANTO ABINAWANTO (Program of Conservation Biology, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia. Jl. Lingkar UI, Depok 16242, West Java, Indonesia)
YASMAN YASMAN (Program of Conservation Biology, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia. Jl. Lingkar UI, Depok 16242, West Java, Indonesia)



Article Info

Publish Date
31 May 2019

Abstract

Abstract. Dharmayanti N, Supriatna J, Abinawanto, Yasman. 2019. Isolation and partial characterization of Alginate from Sargassum polycystum of Banten, Indonesia. Biodiversitas 20: 1776-1785. The utilization of Sargassum polycystum as an alternative alginate source may reduce the dependence on alginate import as currently alginate demands in Indonesia are 100% supplied from overseas. The purpose of this study was to characterize alginate extracted from Sargassum polycystum obtained from three locations with different ecological characteristics. Isolation of alginate was conducted through partial hydrolysis to separate guluronic acid and mannuronic acid followed by freeze-drying and then the parameters were measured qualitatively and quantitatively using FTIR. The results showed that alginate extracted from Sargassum polycystum collected from Lima Island, Ujung Kulon and Binuangeun were 11.48%, 18.62%, and 5.75%, respectively with the viscosity were 35 cP, 62.50 cP, and 81.33 cP, respectively. The test result of partial hydrolysis of alginate showed that guluronic block (GG) in the alginate polymer of Lima Island, Ujung Kulon and Binuangeun were 67.60%, 59.00%, and 41.40%, respectively. These relate to the nature of the gel formed. The alginate from Lima Island tends to be more rigid and less flexible than that from Ujung Kulon and Binuangeun. The findings of this study suggest that there are differences in the concentration of the components of mannuronate and guluronate of Sargassum polycystum across different locations in Banten, Indonesia.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...