Biodiversitas Journal of Biological Diversity
Vol. 20 No. 9 (2019)

The virulence improvement of Beauveria bassiana in infecting Cylas formicarius modulated by various chitin based compounds

triono bagus saputro (Department of Biology, Faculty of Science, Institut Teknologi Sepuluh Nopember. Jl. Arif Rahman Hakim, Keputih, Sukolilo, Surabaya 60111, East Java, Indonesia)
YUSMANI PRAYOGO (Indonesian Legumes and Tuber Crops Research Institute. Jl. Kendal Payak Km. 8 Malang 65101, East Java, Indonesia)
FARADIBA LAZUARDI ROHMAN (Department of Biology, Faculty of Science, Institut Teknologi Sepuluh Nopember. Jl. Arif Rahman Hakim, Keputih, Sukolilo, Surabaya 60111, East Java, Indonesia)
NUR HIDAYATUL ALAMI (Department of Biology, Faculty of Science, Institut Teknologi Sepuluh Nopember. Jl. Arif Rahman Hakim, Keputih, Sukolilo, Surabaya 60111, East Java, Indonesia)



Article Info

Publish Date
13 Aug 2019

Abstract

Abstract. Saputro TB, Prayogo Y, Rohman FL, Alami NH. 2019. The virulence improvement of Beauveria bassiana in infecting Cylas formicarius modulated by various chitin based compounds. Biodiversitas 20: 2486-2493. Sweet potato (Ipomoea batatas L.) is one of alternative carbohydrate source with an important role in food production, animal feed industries, and as raw materials for other derivatives. However, severe losses are constantly occurred in its production due to a notorious pest known as sweet potato weevil (Cylas formicarius). The chemical insecticides commonly used to treat the pest, but have not been efficient in eliminating C.formicarius. Beauveria bassiana is well known as a biological control agent, has been identified to effectively eliminate the pest. The aim of this research is to observe the optimum concentration of several chitin-based compounds in improving the virulence of B. bassiana. The research was conducted by the addition of chitin from various sources - insects, crustaceans and mollusk shells in a growth medium of B. Bassiana. Moreover, conidia produced by B.bassiana was exposed to C.formicarius to investigate the mortality rate of this insect. The results showed four different characteristics of colonies - velvety, wooly, pellicular, and farinaceous. The 1% chitin from Tellina sp, (P9), was found to be the best treatment which increased the growth of the colony diameter attaining 6.7cm in 20 days, increased the conidia viability that reaches 93.5%. In addition, the highest mortality percentage of C. formicarius was 91.67% in P9 at 6 days after infection. Overall, this research gave new potential sources of chitin that can be applied in improving the virulence of B. Bassiana in eliminating C. formicarius.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...