Biodiversitas Journal of Biological Diversity
Vol. 21 No. 4 (2020)

Identification and expression of gonadotrophin hormones in gouramy (Osphronemous gouramy, Lacepède, 1801) under photoperiod manipulations

norman prayogo (Faculty of Fisheries and Marine Science, Universitas Jenderal Soedirman. Jl. Dr. Soeparno, Karangwangkal, Purwokerto Utara, Banyumas 53122, Central Java, Indonesia)
asrul siregar (Faculty of Fisheries and Marine Science, Universitas Jenderal Soedirman. Jl. Dr. Soeparno, Karangwangkal, Purwokerto Utara, Banyumas 53122, Central Java, Indonesia)
purnama sukardi (Faculty of Fisheries and Marine Science, Universitas Jenderal Soedirman. Jl. Dr. Soeparno, Karangwangkal, Purwokerto Utara, Banyumas 53122, Central Java, Indonesia)
yasumasa bessho (Division of Biological Science, Graduate Scholl of Science and Technology, Nara Institute Science and Technology. 8916-5 Takayama, Ikoma, Nara 630-01-01, Japan)



Article Info

Publish Date
10 Mar 2020

Abstract

Abstract. Prayogo NA, Siregar AS, Sukardi P, Bessho Y. 2020. Identification and expression of gonadotrophin hormones in gouramy (Osphronemoous gouramy, Lacepède, 1801) under photoperiod manipulations. Biodiversitas 21: 1365-1372. The environment influences fish reproduction. One of the influential environmental factors is photoperiod that regulates the production of hormones for gonad growth and development, gametogenesis, then the reproductive cycle. This research was conducted to determine the effect of photoperiod on the reproductive performance of gouramy with different photoperiod treatments. The design of this study was four photoperiod treatments consisting of 14L: 10D, 16L: 8D and 18L: 6D and controls. Fish samples were divided into 4 aquariums consisting of nine fish and repetition was conducted on each. Fish were kept under photoperiod treatment for 16 weeks. The observed variable was pituitary activity. Pituitary activity was evaluated by measuring the expression of FSH and LH genes using Real Time PCR. The results showed that gouramy had FSH and LH genes with 483 bp and 506 bp. FSH and LH gene expression increased with long photoperiod exposure (P <0.05). These findings indicate that photoperiods regulate the reproduction of gouramy through the HPG axis and control the production of the hormone Gonadotropin.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...