Biodiversitas Journal of Biological Diversity
Vol. 21 No. 12 (2020)

The detection of plantaricin-encoding genes and their amino acid profiles in Lactobacillus plantarum AKK30 isolated from Indonesian native chicken

ELISABETH LOVIAN ULI BASA (Program of Biology, Graduate Program, Faculty of Mathematics and Natural Sciences, Universitas Indonesia. Jl. Lingkar UI, Depok 16424, West Java, Indonesia)
Abinawanto Abinawanto (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia. Jl. Lingkar UI, Depok 16424, West Java, Indonesia)
A. SOPHIAN (National Agency of Drug and Food Control (BPOM Gorontalo). Jl. Toto Selatan, Bone Bolango 96128, Gorontalo, Indonesia)
H. JULENDRA (Reserach Unit for Natural Products Technology, Indonesian Institute of Sciences. Jl. Raya Wonosari Km 31.5, Playen, Gunung Kidul 55861, Yogyakarta, Indonesia)
A. SOFYAN (Reserach Unit for Natural Products Technology, Indonesian Institute of Sciences. Jl. Raya Wonosari Km 31.5, Playen, Gunung Kidul 55861, Yogyakarta, Indonesia)



Article Info

Publish Date
28 Nov 2020

Abstract

Abstract. Elisabeth LUB, Abinawanto, Sophian A, Julendra H, Sofyan A. 2020. The detection of plantaricin-encoding genes and amino acid profile in Lactobacillus plantarum AKK30 isolated from Indonesian native chicken. Biodiversitas 21: 5792-5799. Plantaricin-encoding genes of lactic acid bacteria (LAB) Lactobacillus plantarum AKK30 which was isolated from Indonesian native chicken has been characterized using DNA sequencing technology. In order to substitute antibiotics for chickens and enhance probiotics in their gut, characterization of the indigenous probiotics in the chicken should be conducted. A potential probiotic in native chicken gut is Lactobacillus plantarum AKK30. Genes that harbor the bacteriocin biosynthesis of the strain were reported for the first time. The bacterium has diverse plantaricin genes, i.e., plnA, plnEF, plnJ, plnK and plnO. According to the dendrogram, the five plantaricin genes were closely related to plnA, plnE, plnF, plnJ, plnK and plnO genes from L. plantarum WCFS1, L. plantarum C11, and L. plantarum V90. Intriguingly, besides various plantaricin-encoding genes, another important probiotic property in L. plantarum AKK30 is cholesterol-lowering activity. Amino acid profiling also indicated proteolysis activity and probiotic potential, for instance, GABA production. Thus, L. plantarum AKK30 may be added in chicken broiler’s diet to provide probiotic benefits.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...