Biodiversitas Journal of Biological Diversity
Vol. 21 No. 3 (2020)

Genetic diversity of local corn (Zea mays) cultivars from South Amarasi, Kupang District, Indonesia by Inter Simple Sequence Repeats marker

Uslan Uslan (Department of Biology Education, Faculty of Teacher Training and Education, Universitas Muhammadiyah Kupang. Jl. KH. Ahmad Dahlan No. 17, Kayu Putih, Oebobo, Kupang City 85111, East Nusa Tenggara, Indonesi)
NUR JANNAH (Department of Biology Education, Faculty of Teacher Training and Education, Universitas Muhammadiyah Kupang. Jl. KH. Ahmad Dahlan No. 17, Kayu Putih, Oebobo, Kupang City 85111, East Nusa Tenggara, Indonesia)



Article Info

Publish Date
26 Feb 2020

Abstract

Abstract. Uslan, Jannah N. 2020. Genetic diversity of local corn (Zea mays) cultivars from South Amarasi, Kupang District, Indonesia by Inter Simple Sequence Repeats marker. Biodiversitas 21: 1208-1214. Corn (Zea mays L.) is one of the most important food crops in Indonesia. However, the studies described their genetic variation is relatively poor. Therefore, the aim of this study was to analyze the genetic diversity of local corn cultivars from South Amarasi, Kupang District, East Nusa Tenggara (NTT), Indonesia using ISSR markers. The sampling was conducted in 4 different sites in Sub-district of Amarasi, Kupang District. A total of 11 corn cultivars from Sub-district of South Amarasi was collected. DNA isolation was performed by using CTAB Method. Clustering analysis was conducted on MSVP 3.2 software. It was shown that all ISSR-primers used (UBC 811, UBC 814 and UBC 824) were successfully produced polymorphic bands and represents the high genetic diversity of the local corn cultivars. The genetic distance index indicated that several corn cultivars from two different populations were geographically unclustered, although there are samples from several populations that have a low genetic distance. The genetic variation index also showed high genetic diversity among the populations. Further research on the exhaustive sample collection was needed to give an insight into the genetic diversity of local corn cultivars (Zea mays L.) from South Amarasi, Indonesia. Please write implementation of this research

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...