Short Communication: Identification and phylogenetic analysis of Corynebacterium diphtheriae isolates from Jakarta, Indonesia based on partial rpoB gene

Andi Yasmon (Department of Microbiology, Faculty of Medicine, Universitas Indonesia/Cipto Mangunkusumo Hospital. Jl.Pegangsaan Timur 16, Cikini, Jakarta Pusat 10320, Jakarta, Indonesia)
YEVA ROSANA (Department of Microbiology, Faculty of Medicine, Universitas Indonesia/Cipto Mangunkusumo Hospital. Jl.Pegangsaan Timur 16, Cikini, Jakarta Pusat 10320, Jakarta, Indonesia)
DONI USMAN (Biomedical Science Graduate Program, Faculty of Medicine, Universitas Indonesia. Jl. Salemba Raya No. 5, Jakarta Pusat 10320, Jakarta, Indonesia)
LUH INTA PRILANDARI (Specialist Program of Clinical Microbiology, Faculty o Medicine, Universitas Indonesia. Jl. Salemba Raya No.5, Jakarta Pusat 10320, Jakarta, Indonesia)
TEGUH SARRY HARTONO (Infectious Disease Hospital Prof. Dr. Sulianti Saroso. Jl. Sunter Permai Raya, Jakarta Utara 14340, Jakarta, Indonesia)

Biodiversitas Journal of Biological Diversity
Vol. 21 No. 7 (2020)

Abstract. Yasmon A, Rosana Y, Usman D, Prilandari LI, Hartono TS. 2020. Short Communication: Identification and phylogenetic analysis of Corynebacterium diphtheriae isolates from Jakarta, Indonesia based on partial rpoB gene. Biodiversitas 21: 3070-3075. Indonesia is a developing country with the greatest potential of diphtheria, so accurate identification is needed to detect C. diphtheriae. The genetic relationship analysis is important to be determined so that this transmittable disease can be prevented. In this study, we reported the identification and phylogenetic analysis of C. diphtheriae based on partial rpoB gene. Twelve samples from throat swabs of diphtheria suspected patients from 2018 to 2019 were used in this study. The result of VITEK 2 ANC-Automated Testing identified that all isolates were C. diphtheriae. However, the identification based on partial rpoB gene analysis showed that only 10 out of 12 isolates were C. diphtheriae, while 2 other isolates were C. argentoratense. Of 10 C. diphtheriae strains/isolates, 2 strains were unique for Indonesia. Thus, it is needed to evaluate the accuracy of VITEK 2 ANC-Automated Testing and to conduct further comprehensive studies on the genetic relationships of C. diphtheriae strains based on multiple virulence genes, multilocus sequence typing and/or whole-genome analysis.