Biodiversitas Journal of Biological Diversity
Vol. 21 No. 5 (2020)

The genetic relationships and Indo-Pacific connectivity of whale sharks (Rhincodon typus) with particular reference to mitochondrial COI gene sequences from Cendrawasih Bay, Papua, Indonesia

Abdul Hamid A. Toha (Department of Fisheries, Faculty of Fisheries and Marine Science, ¬Universitas Papua. Jl. Gunung Salju, Manokwari 98314, West Papua, Indonesia)
MUHAMMAD DAILAMI (Department of Aquaculture, Faculty of Fisheries and Marine Science, Universitas Brawijaya. Jl. Veteran No. 16, Malang 65145, East Java, Indonesia)
SAIFUL ANWAR (Teluk Cenderawasih National Park Office. Jl. Dr. Esau Sesa, Sowi Gunung, Manokwari 98315, West Papua, Indonesia)
JUSWONO B. SETIAWAN (World Wildlife Fund-Indonesia region of Cenderawasih Bay. Rado, Wasior, Teluk Wondama 98362, West Papua, Indonesia)
YUSUP JENTEWO (World Wildlife Fund-Indonesia region of Cenderawasih Bay. Rado, Wasior, Teluk Wondama 98362, West Papua, Indonesia)
IDA LAPADI (Department of Fisheries, Faculty of Fisheries and Marine Science, ¬Universitas Papua. Jl. Gunung Salju, Manokwari 98314, West Papua, Indonesia)
SANNY SUTANTO (Teluk Cenderawasih National Park Office. Jl. Dr. Esau Sesa, Sowi Gunung, Manokwari 98315, West Papua, Indonesia)
RATIH ARYASARI (Program of Biology, Faculty of Biology, Universitas Gadjah Mada. Jl. Teknika Selatan, Sekip Utara, Sleman 55281, Yogyakarta, Indonesia)
AMBARIYANTO AMBARIYANTO (Department of Marine Science, Faculty of Fisheries and Marine Science, Universitas Diponegoro. Jl. Prof. H. Soedarto, Tembalang, Semarang 50275, Central Java, Indonesia)
FERAWATI RUNTUBOI (Department of Marine Science, Faculty of Fisheries and Marine Science, Universitas Papua. Jl. Gunung Salju, Manokwari 98314, West Papua, Indonesia)
HAWIS MADDUPPA (Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, Institut Pertanian Bogor. Jl. Agatis, Dramaga, Bogor 16680, West Java, Indonesia)



Article Info

Publish Date
25 Apr 2020

Abstract

The whale shark (Rhincodon typus) inhabits Cenderawasih Bay at the Birdhead Seascape of Papua in high frequent sightings, but little is known about its genetic characteristics and connectivity. The study was conducted to characterize of the R. typus COI gene fragment from Cenderawasih Bay, in order to accurately compare with the 27 COI gene fragment sequences from the global whale shark deposited in the GenBank. A total of 28 meat samples of whale shark were collected by biopsy punch on a pole spear. The meat samples were extracted to obtain whole genome then amplified and sequenced to obtained COI nucleotides. The determined COI is a 669 bp, comprising of A = 26.5%, T/U = 30.5%, C = 28.3%, dan G = 14.7%. In total, 41 cutting sites of all the sequences ranged in size from 5-7 bp long. One COI single nucleotide polymorphisms and 2 haplotypes were identified. Single site substitution change from T to C for both haplotypes was observed. Overall, the haplotype diversity (Hd= 0.137) and nucleotide diversity (?=0.0002) were relatively low. A different nucleotide composition, number and arrangement of the sequences with the whale shark COI sequences from Cenderawasih Bay compared to COI gene fragment sequences from GenBank were observed. This study shows the availability of sequence of whale shark will contribute to aid further studies of molecular systematics, phylogeography, genetic differentiation, and conservation genetics.

Copyrights © 2020






Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...