Biodiversitas Journal of Biological Diversity
Vol. 21 No. 4 (2020)

Identification Michelia alba barks extract using Gas Chromatography-Mass Spectrometry (GC-MS) and its antifungal properties to inhibit microbial growth

I Made Dira Swantara (Program on Applied Chemistry, Universitas Udayana. Jl. PB. Sudirman, Denpasar 80238, Bali, Indonesia)
I Gusti Agung Gede Bawa (Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Udayana. Kampus Bukit Jimbaran, Badung 80361, Bali, Indonesia)
Dewa Ngurah Suprapta (Laboratory of Biopesticide, Faculty of Agriculture, Universitas Udayana. Jl. PB. Sudirman, Denpasar 80238, Bali, Indonesia)
Kadek Karang Agustina (Department of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Udayana. Jl. PB. Sudirman, Denpasar 80238, Bali, Indonesia)
I Gede Rai Maya Temaja (Laboratory of Biopesticide, Faculty of Agriculture, Universitas Udayana. Jl. PB. Sudirman, Denpasar 80238, Bali, Indonesia)



Article Info

Publish Date
23 Mar 2020

Abstract

Abstract. Swantara IMD, Bawa IGAG, Suprapta DN, Agustina KK, Temaja IGRM. 2020. Identification Michelia alba barks extract using Gas Chromatography-Mass Spectrometry (GC-MS) and its antifungal properties to inhibit microbial growth. Biodiversitas 21: 1541-1550. Fungicides are substances that inhibit the growth or kill the pathogenic fungi. A substance can be categorized as a fungicide if it shows antifungal activity. This activity is resulting from bioactive compounds derived from the secondary metabolites. Michelia alba bark’s extract could inhibit the growth of Curvularia verruculosa, the cause of leaf spot disease on rice. The purpose of this study was to analyze chemical compounds of bark extract which inhibit the growth of C. verruculosa. The extraction of bark metabolites of M. alba was done using methanol as the solvent. The inhibitory test of the extract was carried out by the diffusion well method on Potato Dextrose Agar (PDA) medium. Analysis of chemical compounds of the M. alba extract was carried out in Gas chromatography-mass spectroscopy. The results of inhibitory test found that the M alba bark extract inhibits the C. verruculosa with the inhibition zone as wide as 36 mm in diameter. The majority of chemical compounds identified from the M. alba extract consisted of 10 compounds, namely: Hexadecanoic acid, methyl ester; 4H-tomentosine; 4H-tomentosine (compound isomer 2); 3-hydroxy pregn-4-ene-20-one; Tomentosine; 2-hydroxy tomentosine; Tert-butyl-2-aminophenylcarbamol; 2H-cyclohepta [b] furan-2-one, 3,3a, 4,7,8,8a-hexahydro-7-methyl-3-methylene-6-(2-formil-3-oxobutyl)-; Isoxanthanol and Xanthanol. To conclude, this extract is useful to treat C. verruculosa that causes leaf spot disease on rice.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...