The combination of mare's milk and grape polyphenol extract for treatment of dysbiosis induced by dextran sulfate sodium

Samat Kozhakhmetov (Laboratory of Human Microbiome and Longevity, Center for Life Sciences, National Laboratory Astana, Nazarbayev University. Z05H0P9, Kabanbay Batyr avenue 53, block S1, Nur-Sultan, Kazakhstan)
DMITRIY BABENKO (Research Center Karaganda Medical University. M01K6T3, Gogol street 40, Karagandy, Kazakhstan)
MADIYAR NURGAZIYEV (Laboratory of Human Microbiome and Longevity, Center for Life Sciences, National Laboratory Astana, Nazarbayev University. Z05H0P9, Kabanbay Batyr avenue 53, block S1, Nur-Sultan, Kazakhstan)
ALTYNAY TUYAKOVA (Laboratory of Human Microbiome and Longevity, Center for Life Sciences, National Laboratory Astana, Nazarbayev University. Z05H0P9, Kabanbay Batyr avenue 53, block S1, Nur-Sultan, Kazakhstan)
AYAULYM NURGOZHINA (Laboratory of Human Microbiome and Longevity, Center for Life Sciences, National Laboratory Astana, Nazarbayev University. Z05H0P9, Kabanbay Batyr avenue 53, block S1, Nur-Sultan, Kazakhstan)
NURISLAM MUHANBETGANOV (Laboratory of Human Microbiome and Longevity, Center for Life Sciences, National Laboratory Astana, Nazarbayev University. Z05H0P9, Kabanbay Batyr avenue 53, block S1, Nur-Sultan, Kazakhstan)
LAURA CHULENBAYEVA (Laboratory of Human Microbiome and Longevity, Center for Life Sciences, National Laboratory Astana, Nazarbayev University. Z05H0P9, Kabanbay Batyr avenue 53, block S1, Nur-Sultan, Kazakhstan)
SHYNGGYS SERGAZY (Laboratory of Human Microbiome and Longevity, Center for Life Sciences, National Laboratory Astana, Nazarbayev University. Z05H0P9, Kabanbay Batyr avenue 53, block S1, Nur-Sultan, Kazakhstan)
ALEXANDR GULYAYEV (Laboratory of Human Microbiome and Longevity, Center for Life Sciences, National Laboratory Astana, Nazarbayev University. Z05H0P9, Kabanbay Batyr avenue 53, block S1, Nur-Sultan, Kazakhstan)
TIMUR SALIEV (S.D. Asfendiyarov Kazakh National Medical University. A05H2A6, Tole Bi Street 94, Almaty, Kazakhstan)
ALMAGUL KUSHUGULOVA (Laboratory of Human Microbiome and Longevity, Center for Life Sciences, National Laboratory Astana, Nazarbayev University. Z05H0P9, Kabanbay Batyr avenue 53, block S1, Nur-Sultan, Kazakhstan)

Biodiversitas Journal of Biological Diversity
Vol. 21 No. 5 (2020)

Abstract. Kozhakhmetov S, Babenko D, Nurgaziyev M, Tuyakova A, Nurgozhina A, Muhanbetganov N, Chulenbayeva L, Sergazy S, Gulyayev A, Saliev T, Kushugulova A. 2020. The combination of mare's milk and grape polyphenol extract for treatment of dysbiosis induced by dextran sulfate sodium. Biodiversitas 21: 2275-2280. This study showed the potential of a biological product based on mare's milk and a complex of grape polyphenols to modulate intestinal microflora after dextran sulfate sodium  (DSS)-induced dysbiosis. Rat ulcerative colitis has been developed using intra-gastric administration of 10% DSS solution. To track changes in the structure of the microbiome at all stages of the study, the next-generation sequencing of the 16S rRNA gene section and LotuS conveyor were used. The results of sequencing demonstrated a decrease in biological diversity of microbiota after the induction of colitis, and recovery after 7 days of use of the  (MMGPE). The product induced the structural changes of the microbiome damaged by DSS. Representatives of SCFA producing bacteria increased concentrations of  Prevotella, Alloprevotella, Lactobacillus, Ruminococcaceae, and Blautia.