Biodiversitas Journal of Biological Diversity
Vol. 22 No. 2 (2021)

DNA intensity and genetic diversity of oil palm (Elaeis guineensis) to determine an elite low lipase line

Nina Unzila Angkat (Graduate Program of Agrotechnology, Faculty of Agriculture, Universitas Sumatera Utara. Jl. Prof. A. Sofyan No. 3 Medan, 20155, North Sumatra, Indonesia)
LUTHFI AZIZ MAHMUD SIREGAR (Department of Agrotechnology, Faculty of Agriculture, Universitas Sumatera Utara. Jl. Prof. A. Sofyan No. 3 Medan, 20155, North Sumatra, Indonesia)
Mohammad Basyuni (Department of Forestry, Faculty of Forestry, Universitas Sumatera Utara. Jl. Tri Dharma Ujung No. 1, Medan, 20155, North Sumatra, Indonesia)
Dadang Afandi (Graduate Program of Agrotechnology, Faculty of Agriculture, Universitas Sumatera Utara. Jl. Prof. A. Sofyan No. 3 Medan, 20155, North Sumatra, Indonesia)
INDRA SYAHPUTRA (PT. Socfin Indonesia. Jl. KL Yos Sudarso No. 106, Medan 20115, North Sumatra, Indonesia)



Article Info

Publish Date
27 Jan 2021

Abstract

Abstract. Angkat NU, Siregar LAM, Basyuni M, Afandi D, Syahputra I. 2021. DNA intensity and genetic diversity of oil palm (Elaeis guineensis) to determine an elite low lipase line. Biodiversitas 22: 900-905. The acidification of palm oil due to lipase activity in the mesocarp is assessed under genetic control. Three molecular markers have been established to gauge the lipase gene in oil palm. Lower lipase activity is desired for good quality edible oil. This study aims to identify the genetic diversity by screening groups/families to determine an elite low lipase genotype of oil palm. Genetic diversity and population structure of 15 groups of oil palm were investigated by using three specific markers with GenAlex 6.502 software. Results show that the Polymorphic Informative Content (PIC) value of markers was around 0,985-0,993, which indicates that these markers are effective in determining the diversity of lipase activity in oil palm. Analysis of molecular variance (AMOVA) revealed that genetic diversity varies within individuals (54%), among individuals (31%), and among population (15%). The value of number of alleles (Na), number of effective alleles (Ne), observed heterozygosity (Ho), expected heterozygosity (He), and number of allele migration (Nm) indicate that the genetic diversity in this population is relatively low. Phylogenetic analysis identified two main groups as high lipase and low lipase activity groups based on DNA intensity.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...