Genetic variability in the A microsatellite at SLC11A1 gene and possible implications with innate resistance against brucellosis in Algerian native goats

Hossem SAHRAOUI (Department of Agronomy, University of Setif. El Bez, Sétif 19000, Algeria)
TOUFIK MADANI (Department of Agronomy, University of Setif. El Bez, Sétif 19000, Algeria)
KHALED FANTAZI (National Institute of Agronomic Research. 2 rue les frères OUADEK Hassen Badi, EL-Harrach, Algiers 16200, Algeria)
ASMA CHAOUCH KHOUANE (National Center for Biotechnology Research. Nouvelle Ville Ali Mendjli, UV 03, Constantine 25016, Algeria)
ABDELKADER AMEUR AMEUR (Department of Biology, University of Tlemcen. 22, Rue Abi Ayad Abdelkrim, Tlemcen, Algeria)
PIETRO PASCHINO (Department of Veterinary Medicine, University of Sassari. Via Vienna, 2, Sassari 07100, Italy)
GIUSEPPE MASSIMO VACCA (Department of Veterinary Medicine, University of Sassari. Via Vienna, 2, Sassari 07100, Italy)
SEMIR BECHIR SUHEIL GAOUAR (Department of Biology, University of Tlemcen. 22, Rue Abi Ayad Abdelkrim, Tlemcen, Algeria)
MARIA LUISA DETTORI (Department of Veterinary Medicine, University of Sassari. Via Vienna, 2, Sassari 07100, Italy)

Biodiversitas Journal of Biological Diversity
Vol. 21 No. 12 (2020)

Abstract. Sahraoui H, Madani T, Fantazi K, Chaouch Khouane A, Ameur Ameur A, Paschino P, Vacca GM, Gaouar SBS, Dettori ML. 2020. Genetic variability in the A microsatellite at SLC11A1 gene and possible implications with innate resistance against brucellosis in Algerian native goats. Biodiversitas 21: 5630-5636. Goat rearing is among the major agricultural activities practiced in Algeria. However, brucellosis represents an important threat to the goat sector and to public health. The aim of our work was to characterize the genetic variability of the A microsatellite at the 3' untranslated region (3' UTR) of SLC11A1 gene in Algerian native goat breeds as it was associated with Brucellosis genetic resistance in goat. Genomic DNA samples of 90 goats belonging to the four Algerian native breeds; Mekatia (n = 32), Arbia (n = 30), Dwarf of Kabylia (n = 14) and Mozabit (n = 14) have been analyzed. Polymorphism of region A microsatellite was determined by capillary electrophoresis identifying 8 alleles and 20 genotypes. Genetic parameters were also estimated. Polymorphic information content was PIC = 0.60, the observed and the expected heterozygosity were Ho = 0.569 and He = 0.595, respectively, and the values of FIS, FIT and FST were 0.044, 0.064, and 0.021, respectively. The A microsatellite was highly polymorphic and alleles associated genetic resistance against brucellosis in other breeds or species have been identified. These results open a promising opportunity to implement a genetic improvement program to reduce goat brucellosis spread in Algeria.