Biodiversitas Journal of Biological Diversity
Vol. 22 No. 3 (2021)

Fucoxanthin identification and purification of brown algae commonly found in Lombok Island, Indonesia

yeni sulistiyani (Department of Aquatic Resource Management, Faculty of Fisheries and Marine Science, Universitas Diponegoro. Jl. Prof. H. Soedarto, SH, Tembalang, Semarang 50275, Central Java, Indonesia)
AGUS SABDONO (Department of Marine Science, Faculty of Fisheries and Marine Science, Universitas Diponegoro. Jl. Prof. H. Soedarto, SH, Tembalang, Semarang 50275, Central Java, Indonesia)
NORMA AFIATI (Department of Aquatic Resource Management, Faculty of Fisheries and Marine Science, Universitas Diponegoro. Jl. Prof. H. Soedarto, SH, Tembalang, Semarang 50275, Central Java, Indonesia)
HAERUDDIN HAERUDDIN (Department of Aquatic Resource Management, Faculty of Fisheries and Marine Science, Universitas Diponegoro. Jl. Prof. H. Soedarto, SH, Tembalang, Semarang 50275, Central Java, Indonesia)



Article Info

Publish Date
04 Mar 2021

Abstract

Abstract. Sulistiyani Y, Sabdono A, Afiati N, Haeruddin. 2021. Fucoxanthin identification and purification of brown algae commonly found in Lombok Island, Indonesia. Biodiversitas 22: 1527-1534. Fucoxanthin is a significant carotenoid pigment found in brown algae. Fucoxanthin is the most human-exploited pigment in brown algae due to its health benefits such as antioxidant, anti-diabetic, anti-obesity, anti-cancer, and anti-inflammatory activities. Therefore, fucoxanthin has been mass-produced on an industrial scale as a food supplement. This work focused on identifying, purifying and comparing the fucoxanthin yield diversity at one study site quantitatively. Three brown algae commonly found in Lombok waters have been studied in this work: Sargassum cf. granuliferum, Sargassum polycystum, and Sirophysalis trinodis. Fucoxanthin was successfully identified using HPLC methods, TLC and Spectra absorption analysis. Using HPLC, fucoxanthin was identified at 450 nm wavelength, with a retention time of 3.088-3.095 min. Based on the TLC result, the Rf value of fucoxanthin is 0.46-0.47, while the spectra absorption maxima are 447.6-448.4 nm. The highest fucoxanthin yield was found in S. polycystum, followed by S. cf. granuliferum and Sirophysalis trinodis with 3.01 ± 0.01 mg/g, 2.21 ± 0.002 mg/g, and 0.95 ± 0.01 mg/g, respectively.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...