Biodiversitas Journal of Biological Diversity
Vol. 22 No. 4 (2021)

DNA barcoding of lamp shells (Brachiopoda: Lingula anatina) from Probolinggo, East Java, Indonesia

Reni Ambarwati (Laboratory of Animal Systematics, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Surabaya. Jl. Ketintang, Gayungan, Surabaya 60231, East Java, Indonesia)
Dwi Anggorowati Rahayu (Laboratory of Animal Systematics, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Surabaya. Jl. Ketintang, Gayungan, Surabaya 60231, East Java, Indonesia)
Fida Rachmadiarti (Laboratory of Ecology, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Surabaya. Jl. Ketintang, Gayungan, Surabaya 60231, East Java, Indonesia)
Firas Khaleyla (Laboratory of Animal Structure, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Surabaya. Jl. Ketintang, Gayungan, Surabaya 60231, East Java, Indonesia)



Article Info

Publish Date
23 Mar 2021

Abstract

Abstract. Ambarwati R, Rahayu DA, Rachmadiarti F, Khaleyla F. 2021. DNA barcoding of lamp shells (Brachiopoda: Lingula anatina) from Probolinggo, East Java, Indonesia. Biodiversitas 22: 1764-1774. Lingula anatina is one of brachiopods found in tropical regions, however, the reports on this species from South East Asia is currently limited. This study, therefore, aims to identify the Cytochrome Oxidase subunit I (COI) sequence and phylogenetic of lamp shells from Probolinggo, Indonesia. A total of five samples of lamp shells were collected from Probolinggo, Indonesia, then identified based on morphological characters, and the identification was confirmed using molecular data. Subsequently, molecular characterization and identification were conducted based on 657 bp of COI gene similarity, sequence variation, genetic distance, phylogenetic topology, and BOLD System. According to the results, the Lingula species found in Probolinggo Indonesia was Lingula anatina. The median-joining network and Automatic Barcode Gap Discovery (ABGD) analysis showed this Lingula anatina population from Probolinggo had relatives of 22 haplotypes and divided into six haplogroups. Furthermore, the population belonged to the same haplogroup but had different haplotypes. Thus, this study provides more information for Lingula anatina phylogenetic and haplotype studies.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...