Biodiversitas Journal of Biological Diversity
Vol. 22 No. 11 (2021)

Nitrogen-fixation and phosphate-solubilization bacteria isolated from alluvial and latosol soil paddy field

IKHWANI IKHWANI (Indonesian Center for Food Crops Research and Development. Jl. Merdeka 147, Bogor 16111, West Java, Indonesia)
NONON SARIBANON (Program of Conservation Biology, Department of Biology, Universitas Nasional. Jl. Sawo Manila No. 61, West Jakarta 12520, Jakarta, Indonesia)
TATANG MITRA SETIA (Program of Conservation Biology, Department of Biology, Universitas Nasional. Jl. Sawo Manila No. 61, West Jakarta 12520, Jakarta, Indonesia)
ERNY YUNIARTI (Indonesian Soil Research Institute. Jl. Tentara No. 12, Bogor 16111, West Java, Indonesia)
Jumakir Jumakir (Assesment Institute of Agricultural Technology of Jambi. Jl. Samarinda, Jambi City 36129, Jambi, Indonesia)



Article Info

Publish Date
19 Oct 2021

Abstract

Abstract. Ikhwani, Saribanon N, Setia TM, Yuniarti E, Jumakir. 2021. Nitrogen-fixation and phosphate-solubilization bacteria isolated from alluvial and latosol soil paddy field. Biodiversitas 22: 4722-4730. This study aims to isolate, select and identify molecular characterization of bacteria from alluvial and latosol soil paddy field. This study has been conducted from February-June 2020 in the Soil Biology Laboratory, ICALRRD, Bogor. Alluvial and latosol soil samples were selected from Pusakanagara and Muara experimental gardens, Muara district. The result showed that bacteria growth in N-free media was higher from latosol soil as compared to alluvial soil. The higher nitrogenase activity isolates, could form ethylene gas concentration 1.1.E + 09 µmol/mL/hours, 1.7.E + 09 µmol/mL/hours and 1.3.E + 09 µmol/mL/hours from  alluvial 6, latosol 4, and latosol 9.  Three isolates have great PSB performances, i.e. isolates No. 4.2, 2.1 and 2 could dissolve 14.398 mg l-1, 12.648 mg l-1 and 12.145 mg l-1 of phosphate, from latosol soil. The highest N-fixing and solubilization capacity identified by molecular characterization i.e latosol 4, 4.2 and alluvial 7.1, alluvial-1.11. The phylogeny analyzed the presence of bacterial isolates in two distinct groups. Latosol 3.1 and latosol 4.2 isolates clustered in the same as the genus Lysinibacillus bacteria. The Alluvial 7.1 bacterial isolate showed similarities by Fictibacillus sp. and alluvial 1.2 isolate showed similarities by the genus Bacillus sp.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...