Short Communication: PCR detection of Avibacterium paragallinarum from layers with infectious coryza symptoms in poultry farms of Sleman District, Indonesia

Ima Fauziah (Doctoral Program of Veterinary Science, Faculty of Veterinary Medicine, Universitas Gadjah Mada. Jl. Fauna No. 2, Caturtunggal, Depok, Sleman 55281, Yogyakarta, Indonesia)
Widya Asmara (Department of Microbiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada. Jl. Fauna No. 2, Caturtunggal, Depok, Sleman 55281, Yogyakarta, Indonesia)
Agnesia Endang Tri Hastuti Wahyuni (Department of Microbiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada. Jl. Fauna No. 2, Caturtunggal, Depok, Sleman 55281, Yogyakarta, Indonesia)
Rini Widayanti (Department of Biochemistry, Faculty of Veterinary Medicine, Universitas Gadjah Mada. Jl. Fauna No. 2, Caturtunggal, Depok, Sleman 55281, Yogyakarta, Indonesia)

Biodiversitas Journal of Biological Diversity
Vol. 22 No. 11 (2021)

Abstract. Fauziah I, Asmara W, Wahyuni AETH, Widayanti R. 2021. Short Communication: PCR detection of Avibacterium paragallinarum from layers with infectious coryza symptoms in poultry farms of Sleman District, Indonesia. Biodiversitas 22: 4890-4894. Infectious coryza (IC), caused by Avibacterium paragallinarum, is a contagious and infectious respiratory tract disease that affects the commercial poultry industry. Molecular techniques, such as species-specific PCR, HPG-2 PCR are mostly used for the detection of  A. paragallinarum. The current research was carried out to isolate A. paragallinarum from the layers of infectious coryza signs in Sleman District, special region of Yogyakarta, followed by PCR confirmation of the identified bacteria. Nine field isolates were observed and determined based on their colony and cell morphology. All isolates were characterized biochemically and confirmed with species-specific HPG-2 PCR for A. paragallinarum. Out of 9 isolates, 6 (66.7%) isolates were biochemically identified as A. paragallinarum and confirmed by HPG-2 PCR.