Biodiversitas Journal of Biological Diversity
Vol. 22 No. 12 (2021)

Genomic insight of two indigenous probiotics Lactobacillus plantarum Dad-13 and Lactobacillus plantarum Mut-7 from different origins of Indonesian fermented foods

DIAN ANGGRAINI SUROTO (Department of Food Science and Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada. Jl. Flora 1, Sleman 55281, Yogyakarta, Indonesia)
PRATAMA NUR HASAN (Center for Food and Nutrition Studies, Universitas Gadjah Mada. Jl. Teknika Utara, Sleman 55281, Yogyakarta, Indonesia)
ENDANG SUTRISWATI RAHAYU (University Center of Excellence for Research and Application on Integrated Probiotic Industry, Univeristas Gadjah Mada. Jl. Teknika Utara, Sleman 55281, Yogyakarta, Indonesia)



Article Info

Publish Date
01 Dec 2021

Abstract

Abstract. Suroto DA, Hasan PN, Rahayu ES. 2021. Genomic insight of two indigenous probiotics Lactobacillus plantarum Dad-13 and Lactobacillus plantarum Mut-7 from different origins of Indonesian fermented foods. Biodiversitas 22: 5491-5500. Lactobacillus plantarum Dad-13 and Lactobacillus plantarum Mut-7 are probiotics isolated from traditional fermented foods. In the present study two strains of L. plantarum were sequenced using Illumina NovaSeq PE150 platform and in silico analysis was performed using RAST webserver to unravel the functional metabolic between two strains. The L. plantarum Dad-13 and Mut-7 genome sizes were 3.25 and 3.98 Mb with GC content of them being almost similar i.e. 44.4 and 44.3%, respectively. Two lactobacilli also had some differences in their metabolic genes, particularly in carbohydrate subsystem. L. plantarum Mut-7 had genes related to the metabolisms of plant cell wall components such as dextrin, arabinose, xylose indicated the adaptation to their environmental origins (fermented dried cassava), while those genes were not observed in L. plantarum Dad-13 which was isolated from fermented buffalo’s milk (dadih). Further analysis using BAGEL4 webserver exhibited their different potential to synthesize bacteriocins, L. plantarum Dad-13 was predicted to produce more types of bacteriocins than L. plantarum Mut-7. This investigation revealed that L. plantarum Dad-13 and L. plantarum Mut-7 have a great potential related with their properties as probiotics.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...