Biodiversitas Journal of Biological Diversity
Vol. 23 No. 7 (2022)

Trait selection and screening of Indonesian local rice accessions for iron stress tolerance

KHAIRUNNISA LUBIS (Department of Agrotechnology, Faculty of Agriculture, Universitas Sumatera Utara. Jl. Prof. A. Sofyan No. 3, Medan 20155, North Sumatra, Indonesia)
LISNAWITA LISNAWITA (Department of Agrotechnology, Faculty of Agriculture, Universitas Sumatera Utara. Jl. Prof. A. Sofyan No. 3, Medan 20155, North Sumatra, Indonesia)
FRANS MAATHUIS (Department of Biology, University of York. Heslington, York YO10 5DD, United Kingdom)
IRDA SAFNI (Department of Agrotechnology, Faculty of Agriculture, Universitas Sumatera Utara. Jl. Prof. A. Sofyan No. 3, Medan 20155, North Sumatra, Indonesia)



Article Info

Publish Date
14 Jul 2022

Abstract

Iron (Fe) poisoning in rice causes changes in both morphological and physiological characteristics of the plants, but the stress response is often genotype-dependent. This research used a screening method on nutrient culture media to evaluate 20 local Indonesian genotypes for tolerance to Fe stress by studying the response of root morphology, vegetative growth, and biomass. This research aimed to identify cultivars that were highly resistant to excess Fe from 20 Indonesian local rice accessions. The research was conducted in a greenhouse from May to June 2019 and used a randomized complete block design with two factors and three replications. The first factor consisted of 20 local Indonesian rice accessions. The second factor was the concentration of Fe in the nutrient media (0, 100, 200, and 300 ppm Fe). The results showed that the high Fe concentration in the nutrient solution decreased root growth, vegetative growth, and biomass in a genotype-specific manner. The application of > 200 ppm Fe in the medium resulted in inhibition of plant growth characterized by yellowing and dry leaves, a decrease in the number of root branches, root length, number of tillers, leaf length, and shoot/root biomass. Several tolerant cultivars were discovered, including Inpara 9, Lipigo 2, and Sigambiri Merah, which could be useful in tolerance breeding. Evaluation of all parameters identified shoot dry weight and number of leaves as reliable traits to assess Fe toxicity tolerance in rice.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...