Biodiversitas Journal of Biological Diversity
Vol. 23 No. 5 (2022)

Short Communication: First report of Nectria haematococca causing a moler disease on shallots in West Nusa Tenggara, Indonesia

MARIANI MARIANI (Doctoral Program in Agricultural Science, Faculty of Agriculture, Universitas Udayana. Jl. PB. Sudirman, Dangin Puri Klod, Denpasar 80234, Bali, Indonesia)
DEWA NGURAH SUPRAPTA (Doctoral Program in Agricultural Science, Faculty of Agriculture, Universitas Udayana. Jl. PB. Sudirman, Dangin Puri Klod, Denpasar 80234, Bali, Indonesia)
I MADE SUDANA (Doctoral Program in Agricultural Science, Faculty of Agriculture, Universitas Udayana. Jl. PB. Sudirman, Dangin Puri Klod, Denpasar 80234, Bali, Indonesia)
I GEDE RAI MAYA TEMAJA (Doctoral Program in Agricultural Science, Faculty of Agriculture, Universitas Udayana. Jl. PB. Sudirman, Dangin Puri Klod, Denpasar 80234, Bali, Indonesia)
I MADE SUDANTHA (Department of Agroecotechnology, Faculty of Agriculture, Universitas Mataram. Jl. Majapahit No.62, Gomong, Selaparang, Mataram 83115, Nusa Tenggara Barat, Indonesia)



Article Info

Publish Date
20 May 2022

Abstract

Abstract. Mariani, Suprapta DN, Sudana IM, Temaja IGRM, Sudantha IM. 2022. Short Communication: First report of Nectria haematococca causing a moler disease on shallots in West Nusa Tenggara, Indonesia. Biodiversitas 23: 2768-2774. Moler disease caused by Nectria haematococca is the main disease of shallots in West Nusa Tenggara, Indonesia. Moler disease caused significant yield loss and even crop failure. The purpose of this study was to isolate and identify the pathogen causing moler disease on shallots. This research was conducted from February 2018 to November 2019. Survey was conducted at 13 shallot planting locations in West Nusa Tenggara, namely: Ngali, Ncera, Cenggu, Renda, Sajang, Rensing, Dasan Baru, Batu Layar, Anyar, Sembalun, Jerowaru, Batujai and Sakuru Village. Ten infected plants were taken, at each observation point, then put in a labeled plastic bag and brought to the laboratory for isolation purposes. The results showed that pathogen causing moler disease on shallot was isolated NTB2018. On PDA isolate NTB2018 produced white color colony, the mycelium growth direction was sideways with smooth mycelium structure, sideways growth direction of mycelium and fine structure mycelium. The fungus produced insulated mycelium consisting of macroconidia and microconidia as asexual spores and ascospores as sexual spores. Based on analysis of 18S rRNA gene, isolate NTB2018 DNA fragment of ±569 bp.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...