Assessment of in vitro activities and chemical profiling of Senecio hoggariensis growing in Algerian Sahara

YASMINE ARAB (Laboratory of Biomolecules and Plant Breeding, Life Science and Nature Department, Faculty of Exact Science and Life Science and Nature, University of Larbi Ben Mhidi Oum El Bouaghi. Oum El Bouaghi, Algeria)
BIHTER SAHIN (Department of Chemistry, Faculty of Science, Mugla Sitki Kocman University. Mugla 48000, Turkey)
OZGUR CEYLAN (Food Quality Control and Analysis Program, Ula Ali Kocman Vocational School, Mugla Sitki Kocman University. Mugla 48147, Turkey)
AMAR ZELLAGUI (Laboratory of Biomolecules and Plant Breeding, Life Science and Nature Department, Faculty of Exact Science and Life Science and Nature, University of Larbi Ben Mhidi Oum El Bouaghi. Oum El Bouaghi, Algeria)
OZGE TOKUL OLMEZ (Department of Chemistry, Faculty of Science, Mugla Sitki Kocman University. Mugla 48000, Turkey)
SELCUK KUCUKAYDIN (Department of Medical Services and Techniques, Koycegiz Vocational School of Health Services, Mugla Sitki Kocman University, Mugla 48800, Turkey)
ALFRED NGENGE TAMFU (Departement of Chemical Engineering, School of Chemical Engineering and Mineral Industries. University of Ngaoudere. 454 Ngaoudere, Cameroon)
MEHMET OZTURK (Department of Chemistry, Faculty of Science, Mugla Sitki Kocman University. Mugla 48000, Turkey)
NOUREDDINE GHERRAF (Laboratory of Natural Resources and Management of Sensitive Environments, University of Larbi Ben Mhidi. Oum El Bouaghi, Algeria)

Biodiversitas Journal of Biological Diversity
Vol. 23 No. 7 (2022)

Abstract. Arab Y, Sahin B, Ceylan O, Zellagui A, Olmez OT, Kucukaydin S, Tamfu AN, Ozturk M, Gherraf N. 2022. Assessment of in vitro activities and chemical profiling of Senecio hoggariensis growing in Algerian Sahara. Biodiversitas 23: 3498-3506. The in vitro antioxidant, anticholinesterase, tyrosinase inhibitory, antibiofilm, and anti-quorum sensing activities of the ethyl acetate extract of Senecio hoggariensis,growing in Algerian Sahara, were studied along with its chemical constituents using HPLC-DAD. The chromatographic analysisunveiled seven phenolic compounds, including p-coumaric acid as a major component. Additionally, the extract showed moderate DPPH radical scavenging activity, compared to known standards. At 200 ?g/mL, the extract disclosed equitable acetylcholinesterase (AChE), butyryl-cholinesterase (BChE) and tyrosinase inhibition rates with respective values of 37.01±1.48%, 18.87±4.18% and 45.99±1.81%. Likewise, the extract exhibited a good antibiofilm activity against Candida albicans ATCC 10239 biofilm production with an inhibition ratio of 59.56±0.40%, at 50µg/mL. The anti-quorum sensing by QS-regulated violacein pigment production inhibition test was determined using Chromobacterium violaceum CV026 and CV12472. The swarming motility inhibition assay was determined using Pseudomonas aeruginosa PA01. It is evident from the findings that Senecio hoggariensis could be considered potential antioxidant, anti-QS, and antibiofilm compounds. However, the origin of anti-biofilm and anti-quorum sensing activities of the ethyl acetate extract could be revealed by further studies on the mechanism of action of active compounds that can be isolated via activity-guided fractionation.