Biodiversitas Journal of Biological Diversity
Vol. 23 No. 8 (2022)

Short Communication: Determination of crude oil degradation efficiency of biofilm producing bacteria isolated from oil contaminated site

DE-HWA KHOO (Environmental and Life Sciences Programme, Faculty of Science, Universiti Brunei Darussalam. Jl. Tungku Link BE1410, Brunei Darussalam)
POOJA SHIVANAND (Environmental and Life Sciences Programme, Faculty of Science, Universiti Brunei Darussalam. Jl. Tungku Link BE1410, Brunei Darussalam)
HUSSEIN TAHA (Environmental and Life Sciences Programme, Faculty of Science, Universiti Brunei Darussalam. Jl. Tungku Link BE1410, Brunei Darussalam)



Article Info

Publish Date
07 Aug 2022

Abstract

Abstract. Khoo DH, Shivanand P, Taha H. 2022. Short Communication: Determination of crude oil degradation efficiency of biofilm producing bacteria isolated from oil contaminated site. Biodiversitas 23: 4138-4143. Microbes have gained much attention for their application in the bioremediation of petroleum contaminated areas, and biofilm producing bacteria are considered potential candidates for this purpose. The aim of this study was to screen six oil degrading bacterial strains for their ability to form biofilm, and to measure the crude oil degradation efficiency of selected strains. It was found that only two strains, Micrococcus sp. UBDBH15 and Pseudomonas sp. UBDBH26 were categorized as strong biofilm producers, with the latter showing the highest amount of biofilm formed. Under the conditions tested, Pseudomonas sp. UBDBH26 was also found to have the highest degradation rate (0.0047 g/day) with a significant degradation of 7.07 ± 3.02 and 11.38 ± 2.93 % of crude oil after 7 and 14 days respectively. Micrococcus sp. UBDBH15 had a lower rate of degradation (0.0033 g/day), resulting in a non-significant degradation. However, in comparison with a non-biofilm producer, Enterobacter sp. UBDBH06, this study suggested that biofilm might enhance the degradation of oil, but further studies are needed to confirm this assumption.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...