Biodiversitas Journal of Biological Diversity
Vol. 23 No. 9 (2022)

Chromatography analysis, in vitro antioxidant and antibacterial activities of essential oil of Artemisia herba-alba Asso of Boussaâda, Algeria

MOUNIRA KADRI (Department of Biology, Faculty of life sciences and Nature, University Echahid Hamma Lakhdar. El-Oued, Algeria)
ABDELOUAHAB YAHIA (Laboratory of Natural Sciences and Materials (LSNM), Abdelhafid Boussouf University Center. Mila, Algeria)
SANA GOUBI (Department of Biology, Faculty of life sciences and Nature, University Echahid Hamma Lakhdar. El-Oued, Algeria)
NOUR ELHOUDA MEKHEDMI (Department of Biology, Faculty of life sciences and Nature, University Echahid Hamma Lakhdar. El-Oued, Algeria)
MEHDI SELMANE (Department of Biology, Faculty of life sciences and Nature, University Echahid Hamma Lakhdar. El-Oued, Algeria)
AHMED ELKHALIFA CHEMSA (Laboratory of Biodiversity and Application of Biotechnology in Agriculture, University of Echahid Hamma Lakhdar. El-Oued, Algeria)



Article Info

Publish Date
02 Sep 2022

Abstract

Abstract. Kadri M, Yahia A, Goubi S, Mekhedmi NE, Selmane M, Chemsa AE. 2022. Chromatography analysis, in vitro antioxidant and antibacterial activities of essential oil of Artemisia herba-alba Asso of Boussaâda, Algeria. Biodiversitas 23: 4424-4431. Artemisia herba-alba Asso (Asteraceae family) is widespread in the semi-arid and arid steppes of North Africa. This plant is used for traditional treatment. The present study aims to investigate the chemical composition, antioxidant and antimicrobial activities of A. herba-alba essential oil found in southwest Algeria (Boussaâda region). Artemisia herba-alba essential oil was obtained by hydrodistillation method, and its chemical composition was identified by using GC/MS analysis. In addition, the antioxidant activity of the extracted essential oil was determined using the DPPH assay. The antimicrobial activity of the essential oil was determined by the agar disc diffusion method. The essential oil extracted from the aboveground portions by hydrodistillation was analyzed by GC/MS. 38 components were identified, making up 69.37% of the oil, the most important of which is thujone (9.875%), camphor (3.762%), cis-p-menthadien-1-of (3.572%), and isoborneol (2.334%). The observed IC50 values of the DPPH assay were 7.31 ± 0.088 mg/mL. On the other hand, this oil was active against all strains tested, this activity varied from 12.77 ± 0.510 mm on Listeria innocua CIP 74915. These results demonstrate that the plant tested could be a potential source of natural antioxidants and antimicrobial agents.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...