Antibiotic sensitivity profile of multidrug-resistant (MDR) Escherichia coli isolated from dairy cow's milk in Probolinggo, Indonesia

AGUS WIDODO (Doctoral Program in Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia)
MIRNI LAMID (Department of Animal Husbandry, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia)
MUSTOFA HELMI EFFENDI (Department of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia.)
ASWIN RAFIF KHAIRULLAH (Doctoral Program in Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia)
KATTY HENDRIANA PRISCILIA RIWU (Doctoral Program in Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia)
LITA RAKHMA YUSTINASARI (Joint Graduate School, Veterinary Anatomy Laboratory, Faculty of Veterinary Medicine, Yamaguchi University. 1677-1 Yoshida, Yamaguchi 753-8511, Japan)
SHENDY CANADYA KURNIAWAN (Master program of Animal Sciences, Department of Animal Sciences, Specialisation in Molecule, Cell and Organ Functioning, Wageningen University and Research. Wageningen 6708 PB, Netherlands)
ARIF NUR MUHAMMAD ANSORI‬ (Professor Nidom Foundation. Jl. Wisma Permai Tengah I Blok AA-2, Mulyorejo, Surabaya 60115, East Java, Indonesia)
OTTO SAHA MARTUA SILAEN (Doctoral Program in Biomedical Science, Faculty of Medicine, Universitas Indonesia. Jl. Salemba Raya No. 6 Senen, Jakarta 10430, Indonesia)
FIDI NUR AINI EKA PUJI DAMEANTI (Microbiology and Immunology Laboratory, Faculty of Veterinary Medicine, Universitas Brawijaya. Jl. Veteran, Ketawanggede, Lowokwaru, Malang 65145, East Java, Indonesia)

Biodiversitas Journal of Biological Diversity
Vol. 23 No. 10 (2022)

Abstract. Widodo A, Lamid M, Effendi MH, Khairullah AR, Riwu KHP, Yustinasari LR, Kurniawan SC, Ansori ANM, Silaen OSM, Dameanti FNAEP. 2022. Antibiotic sensitivity profile of multidrug-resistant (MDR) Escherichia coli isolated from dairy cow's milk in Probolinggo, Indonesia. Biodiversitas 23: 4971-4976. The presence of resistant bacteria in animal products such as milk can be a new threat because it is directly related to the human food chain. Resistant Escherichia coli has been widely studied and detected in farms in developing countries. The aim of present study was to determine the antibiotic resistance profile of E. coli bacteria from dairy cows taken during the milking process from several dairy farms in Probolinggo district, Indonesia. A total of 150 milk samples were obtained from farms and E. coli was isolated and identified on Eosin methylene blue (EMB) media and biochemical test, such as Triple Sugar Iron Agar (TSIA) and indole test, methyl red test, Voges-Proskauer test, and citrate test (IMViC) were also performed. The antibiotic sensitivity profile was screened using the Kirby-Bauer test and results were interpreted according to the CLSI standard. The results showed that 124/150 (82.67%) E. coli bacteria exhibited highest percentage of antibiotic resistance to tetracycline (13.71%), streptomycin (9.68%), trimethoprim (8.87%), chloramphenicol (0.87%), and aztreonam (1.61%). A total of 9/124 (7.26%) E. coli isolates were detected as multidrug-resistant (MDR) and 1/9 (0.81%) E. coli isolate was suspected as extended spectrum ?-lactamase (ESBL) bacteria which was resistant to aztreonam antibiotic. Thus, the threat of multidrug-resistant (MDR) E. coli can come from milk which can affect public health.