Exploring the potency of Streptomyces koyangensis strain SHP 9-3 isolated from the soil of Enggano Island (Indonesia) as an antibacterial source

DELVI SARA JIHAN PAHIRA (Doctoral Program, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada. Jl. Farmako, Sekip Utara, Sleman 55281, Yogyakarta, Indonesia)
EMA DAMAYANTI (Research Center for Food Technology and Processing, National Research and Innovation Agency. Jl. Yogyakarta-Wonosari Km. 31.5, Gunung Kidul 55861, Yogyakarta, Indonesia)
PUSPITA LISDIYANTI (Research Center for Biosystematic and Evolution, Research Organization for Life Sciences and Environment, National Research and Innovation Agency. Jl. Raya Jakarta-Bogor Km 46, Cibinong, Bogor 16911, West Java, Indonesia)
MUSTOFA MUSTOFA (Department of Pharmacology and Therapy, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada. Jl. Farmako, Sekip Utara, Sleman 55281, Yogyakarta, Indonesia)
TRIANA HERTIANI (Indonesian Biofilm Research Collaboration Centre. Jl. Farmako, Sekip Utara, Sleman 55281, Yogyakarta, Indonesia)

Biodiversitas Journal of Biological Diversity
Vol. 24 No. 3 (2023)

Abstract. Pahira DSJ, Damayanti E, Lisdiyanti P, Mustofa, Hertiani T. 2023. Exploring the potency of Streptomyces koyangensis strain SHP 9-3 isolated from the soil of Enggano Island (Indonesia) as an antibacterial source. Biodiversitas 24: 1460-1466. Streptomyces is a Gram-positive bacterium with the potential a produce beneficial antimicrobials. This study aimed to determine the antimicrobial activity, morphological characteristics, cytotoxicity, and chemical profile of S. koyangensis SHP 9-3. This bacterium was isolated from Enggano Island, Indonesia, and the morphological characteristics were determined using a Scanning Electron Microscope (SEM). Cultivation was conducted in the International Streptomyces Project (ISP-2) media using baffled flasks and a shaker incubator. The secondary metabolites were conducted on the extract against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans by a twofold dilution method. Concurrently, cytotoxicity testing was done on Vero cells. The extract’s chemical profile was analyzed using LC-MS/MS. The results showed a prominent antibacterial activity as the extract inhibited the growth of S. aureus and C. albicans at 7.81 µg/mL. However, a growth inhibition against E. coli was observed at 250 µg/mL and P. aeruginosa at 31.25 µg/mL. The cytotoxic test showed the IC50 value on Vero cells was 5,015 µg/mL, indicating that the extract was nontoxic against Vero cells. The chemical profile of the extract by LC-MS/MS revealed the presence of five major components, i.e., cyclo (Pro-Val) (C10H16N2O2), kuraramine (C12H18N2O2), 1,4-diphenyl-1-pentanone (C17H18O), (3S,4S)-4-amino-3-hydroxy-6-methyl heptanoic acid (C21H42N2O7), and 2-ethyl-8-methyl-2,8-diazaspiro (4,5) decane-1,3-dione (C11H18N2O2).