Biodiversitas Journal of Biological Diversity
Vol. 24 No. 1 (2023)

Enzyme-producing symbiotic bacteria in gastropods and bivalves molluscs: Candidates for bioindustry materials

WILIS ARI SETYATI (Department of Marine Science, Faculty of Fisheries and Marine Science, Universitas Diponegoro. Jl. Prof. H. Soedarto, S.H, Semarang 50275, Central Java, Indonesia)
DELIANIS PRINGGENIES (Department of Marine Science, Faculty of Fisheries and Marine Science, Universitas Diponegoro. Jl. Prof. H. Soedarto, S.H, Semarang 50275, Central Java, Indonesia)
NIRWANI SOENARDJO (Department of Marine Science, Faculty of Fisheries and Marine Science, Universitas Diponegoro. Jl. Prof. H. Soedarto, S.H, Semarang 50275, Central Java, Indonesia)
RINI PRAMESTI (Department of Marine Science, Faculty of Fisheries and Marine Science, Universitas Diponegoro. Jl. Prof. H. Soedarto, S.H, Semarang 50275, Central Java, Indonesia)



Article Info

Publish Date
18 Jan 2023

Abstract

Abstract. Setyati WA, Pringgenies D, Soenardjo N, Pramesti R. 2023. Enzyme-producing symbiotic bacteria in gastropods and bivalves molluscs: candidates for bioindustry materials. Biodiversitas 24: 20-25. Biotechnology plays a vital role in modern civilization because almost all aspects of human life have benefited from the development of biotechnological applications, especially in the bioindustry. Therefore, the present study aims to obtain bacterial isolates from molluscs and identify those with the potential to produce protease, amylase, and cellulase enzymes using bimolecular methods and bacterial biochemical analysis. The samples were collected in April 2022 from the coastal waters of Krakal, Special Region of Yogyakarta, Indonesia. The symbiotic bacteria were isolated, and screened for different enzymatic activity. The molecular method (16S rRNA gene sequence) was used to identify acterial isolates with the highest potential for enzymatic activity. Biochemical analysis of isolates that are potential candidates for industrial materials was carried out using the thin-Layer Chromatography (TLC) method to detect the compounds produced by bacteria. The results showed that the bacterial isolates GS 1-4, GS 2-1, and GS 2-12 had similarity with Alcaligenes faecalis (99.83%), Alcaligenes faecalis (99.74%) and Alcaligenes aquatilis (98.51%) respectively. The three isolates showed the potent enzymatic activity and contained alcohol, amines, aldehyde, ketone and conjugated aromatics, as well as amino acids for potential candidates of bioindustry materials.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...