Biodiversitas Journal of Biological Diversity
Vol. 24 No. 5 (2023)

Bioprospection of single and a consortium endophytic bacteria of suboptimal field rice isolates as biocontrol of rice bacterial leaf blight pathogens

HERU ADI DJATMIKO (Faculty of Agriculture, Universitas Jenderal Soedirman. Jl. Dr. Soeparno No. 63, Purwokerto Utara, Banyumas 53122, Central Java, Indonesia)
NUR PRIHATININGSIH (Faculty of Agriculture, Universitas Jenderal Soedirman. Jl. Dr. Soeparno No. 63, Purwokerto Utara, Banyumas 53122, Central Java, Indonesia)
PUJI LESTARI (Faculty of Mathematics and Natural Sciences, Universitas Jenderal Soedirman. Jl. Dr. Soeparno No. 61, Purwokerto Utara, Banyumas 53122, Central Java, Indonesia)



Article Info

Publish Date
27 May 2023

Abstract

Abstract. Djatmiko HA, Prihatiningsih N, Lestari P. 2023. Bioprospection of single and a consortium endophytic bacteria of suboptimal field rice isolates as biocontrol of rice bacterial leaf blight pathogens. Biodiversitas 24: 2710-2715. Rice root endophytic bacteria isolated from suboptimal land have the potential to control leaf blight by Xanthomonas oryzae pv. oryzae (Xoo). The aim of this research was: 1) to test the potential of single and consortium endophytic bacteria single and consortium to control Xoo in vitro, 2) to know the antibiosis mechanism of this endophytic bacteria and 3) to evaluate the biochemical character of the single endophytic bacteria as an antagonist feature. The completely randomized design with 5 single and 1 consortium treatment, 4 replications was used as a research method. The inhibition was carried out using a diffusion method of 108 cfu/mL endophytic bacterial suspension. Filter paper 6 mm diameter had been dripped 10 µL suspension of endophytic bacteria, then it placed 3 spots on NA that had been poured with Xoo. The variable observed was the inhibition zone of endophytic bacteria, the inhibition mechanism, and the character as antagonist produces protease; and siderophore. The results showed that both single and consortium endophytic bacteria could potentially suppress Xoo in-vitro, and the best inhibition was the consortium with an inhibitory area of ??62.74 mm2 and the bacteriostatic mechanism. The consortium's antibiosis index was 1.49, it can produce protease and siderophore with clear zone and orange color features. The endophytic bacteria consortium has the potential as a biopesticide to be developed as an alternative to control rice bacterial leaf blight.

Copyrights © 2023






Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...