Biodiversitas Journal of Biological Diversity
Vol. 24 No. 5 (2023)

Bacteriocin-producing Pediococcus acidilactici BAMA 15 isolated from "naniura" traditional foods in North Sumatra, Indonesia

SUBANG AINI NASUTION (Doctoral Program of Medicine, Faculty of Medicine, Universitas Prima Indonesia. Jl. Sampul No. 4, Kampus 5, Medan 20118, North Sumatra, Indonesia)
EDY FACHRIAL (Faculty of Medicine, Universitas Prima Indonesia. Jl. Sampul No. 4, Kampus 5, Medan 20118, North Sumatra, Indonesia)
CHRISMIS NOVALINDA GINTING (Faculty of Medicine, Universitas Prima Indonesia. Jl. Sampul No. 4, Kampus 5, Medan 20118, North Sumatra, Indonesia)
LIENA LIENA (Faculty of Medicine, Universitas Prima Indonesia. Jl. Sampul No. 4, Kampus 5, Medan 20118, North Sumatra, Indonesia)
FINNA PISKA (Department of Pharmacy, Universitas Prima Indonesia. Jl. Sampul No. 4, Kampus 5, Medan 20118, North Sumatra, Indonesia)



Article Info

Publish Date
01 Jun 2023

Abstract

Abstract. Nasution SA, Fachrial E, Ginting CN, Liena, Piska F. 2023. Bacteriocin-producing Pediococcus acidilactici BAMA 15 isolated from "naniura" traditional foods in North Sumatra, Indonesia. Biodiversitas 24: 2830-2835. Lactic Acid Bacteria (LAB) have beneficial properties for their hosts, potentially promoting health and balancing the normal gut flora. Meanwhile, bacteriocin is a bacteriostatic polypeptide used as a food preservative. LAB are generally produced from fermented products, such as dengke naniura, a typical Toba Batak food. Therefore, it is estimated that the LAB present in this food has the potential as a probiotic and produces bacteriocin, an antimicrobial polypeptide compound. The purpose of this study was to identify the LAB species found in dengke naniura and to characterize the bacteriocin generated by them. The first study stages included the isolation and biochemical and morphological characterization of LAB. Nine LAB isolates (BAMA 10-11, BAMA 15-20, and BAMA 25) were isolated from the samples. The best strain for use as a probiotic was Pediococcus acidilactici BAMA 15 because its antimicrobial activity against E. coli and S. aureus was 6.44 and 7.53 mm, respectively. The bacteriocin produced has a molecular weight estimated to be ?12 kDa.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...