Biodiversitas Journal of Biological Diversity
Vol. 24 No. 11 (2023)

Potential of endophytic yeasts as biocontrol of Phytophthora capsici, causative agent of foot rot disease on black pepper

DIAN SAFITRI (Faculty of Animal and Agricultural Sciences, Universitas Diponegoro. Jl. Prof. Sudarto, Tembalang, Semarang 50275, Central Java, Indonesia)
SURYO WIYONO (Department of Plant Protection, Faculty of Agriculture, Institut Pertanian Bogor. Jl. Kamper, Kampus IPB Darmaga, Bogor 16680, West Java, Indonesia)
ENDANG DWI PURBAJANTI (Faculty of Animal and Agricultural Sciences, Universitas Diponegoro. Jl. Prof. Sudarto, Tembalang, Semarang 50275, Central Java, Indonesia)



Article Info

Publish Date
16 Nov 2023

Abstract

Abstract. Safitri D, Wiyono S, Purbajanti ED. 2023. Potential of endophytic yeasts as biocontrol of Phytophthora capsici, causative agent of foot rot disease on black pepper. Biodiversitas 24: 5847-5853. Phytophthora capsici is the main pathogen of foot rot disease of black pepper. This disease contributes to a significant decrease in black pepper productivity. Conventional disease management strategies usually require the use of chemical pesticides, which can have harmful environmental and human health consequences. Introducing biological agents such as endophytic yeasts for controlling foot rot disease has minimal effect on human health and is sustainable. One technique to control foot rot disease is to use biological agents such as endophytic yeasts. The aim of this research was to find yeast against Phytophthora capsici. The methods used included (i) study development and identification of endophytic yeast and (ii) in vitro and in vivo antagonism screening of yeast against P. capsici. The results showed that 49 endophytic yeasts were isolated from the leaves, fruits and roots of three black pepper lines. Five isolates had selected based on the highest antibiosis in antagonism test by in vitro: END6, END21, END66, END87, and END14. Isolate END87 had a mycelium inhibition rate of 64.55%, and a low level of inhibition was found in isolate 21, which had a mycelium size of 42.81%. The molecular characterization based on the sequence partial of NL1 and NL4. Moreover, 5 isolates of endophytic yeast (END6, END21, END66, END87, and END14) were identified as Rhodotorula mucilaginosa, Hannaella oryzae, Cryptococcus tephrensis.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...