Genetic variation and phylogenetic position of Taxus sumatrana (Miq.) de Laub. (Taxaceae) in Sumatra, Indonesia based on trnL-trnF region

IRSYAD KAMAL (Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Walisongo Semarang. Jl. Prof. Dr. Hamka (Kampus II), Ngaliyan, Semarang 50185, Central Java, Indonesia)
NAWWALL ARROFAHA (Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Syarif Hidayatullah Jakarta. Ir. H. Juanda Street No. 95, Ciputat, South Tangerang 15412, Banten, Indonesia)
SUSILA SUSILA (Research Centre for Biosystematics and Evolution, National Agency for Research and Innovation. Jl. Raya Bogor km 46, Cibinong 16911, West Java, Indonesia)
KUSUMADEWI SRI YULITA (Research Centre for Ecology and Ethnobiology, National Agency for Research and Innovation. Jl. Raya Bogor km 46, Cibinong 16911, West Java, Indonesia)
HENTI HENDALASTUTI RACHMAT (Research Centre for Ecology and Ethnobiology, National Agency for Research and Innovation. Jl. Raya Bogor km 46, Cibinong 16911, West Java, Indonesia)
ARIDA SUSILOWATI (Faculty of Forestry, Universitas Sumatera Utara. Jl. Padang Bulan, Medan 20155, North Sumatra, Indonesia)

Biodiversitas Journal of Biological Diversity
Vol. 24 No. 9 (2023)

Abstract. Kamal I, Arrofaha N, Susila, Yulita KS, Rachmat HH, Susilowati A. 2023. Genetic variation and phylogenetic position of Taxus sumatrana (Miq.) de Laub. (Taxaceae) in Sumatra, Indonesia based on trnL-trnF region. Biodiversitas 24: 5155-5166. Taxus sumatrana (Miq.) de Laub. is a synonym of T. wallichiana Zucc. by some authors based on morphological characters and its distribution. This species is a medicinal plant and contains taxol as an anti-cancer with slow natural regeneration rate and increasing threats to their existence. This study aimed to determine the genetic diversity and the phylogenetic analysis using trnL-trnF for T. sumatrana growing in Sumatra, Indonesia. The total of 37 individuals from three natural populations of T. sumatrana were studied, consisting of the current new collection from Toba - North Sumatra and previously studied population of Dempo - South Sumatra and Sungai Penuh, Kerinci Seblat National Park - Jambi. The Toba population did not show variation at individual level within the population as similar results were obtained from the previous study. However, one indel (- and A) was found at 217 bp from all three T. sumatrana populations from Sumatra, Indonesia. When the sequences were aligned with the reference sequence from GenBank, single base substitutions were found at 344, 478,722, and 757 bp consisting of one indel and three single nucleotide polymorphisms (SNPs). Phylogeny tree showed that T. sumatrana from Sumatra population clustered together with T. mairei and T. wallichiana var. mairei and later combined with other T. sumatrana available in NCBI.