Biodiversitas Journal of Biological Diversity
Vol. 24 No. 10 (2023)

Amplicon-based sequencing revealed potential microbiologically influenced corrosion in the interim storage for spent fuel of RSG-GAS, Serpong, Indonesia

IRAWAN SUGORO (Research Center for Radiation Process Technology, National Research and Innovation Agency. Jl. Lebak Bulus Raya No. 49, Cilandak 12440, Jakarta, Indonesia)
DIANNISA SYAHWA RAHMA FADILA (Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Syarif Hidayatullah. Jl. Ir H. Juanda No.95, Ciputat, Ciputat Timur, South Tangerang 15412, Banten, Indonesia)
MEGGA RATNASARI PIKOLI (Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Syarif Hidayatullah. Jl. Ir H. Juanda No.95, Ciputat, Ciputat Timur, South Tangerang 15412, Banten, Indonesia)
FERI EKO HERMANTO (Biomolecular and Bioinformatics Research Center, Indonesia Institute of Bioinformatics. Perum Sarimadu II B3 No. 09 Pakisaji, Malang 65162, East Java, Indonesia)
FIRDAUS RAMADHAN (Institut Sains dan Teknologi Nasional. Jl. Moch. Kahfi II, Srengseng Sawah, Jagakarsa, South Jakarta 12630, Jakarta, Indonesia)
SYALWA ERSADIWI SHALSABILLA (Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Syarif Hidayatullah. Jl. Ir H. Juanda No.95, Ciputat, Ciputat Timur, South Tangerang 15412, Banten, Indonesia)
ADE CICI (Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Syarif Hidayatullah. Jl. Ir H. Juanda No.95, Ciputat, Ciputat Timur, South Tangerang 15412, Banten, Indonesia)
MOHAMMAD SYAMSUL RIJAL (Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Syarif Hidayatullah. Jl. Ir H. Juanda No.95, Ciputat, Ciputat Timur, South Tangerang 15412, Banten, Indonesia)
DYAH SULISTYANI RAHAYU (Research Center for Radiation Process Technology, National Research and Innovation Agency. Jl. Lebak Bulus Raya No. 49, Cilandak 12440, Jakarta, Indonesia)
MARHAENI JOKO PUSPITO (Research Center for Radiation Process Technology, National Research and Innovation Agency. Jl. Lebak Bulus Raya No. 49, Cilandak 12440, Jakarta, Indonesia)
DINDA RAMA HARIBOWO (Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Syarif Hidayatullah. Jl. Ir H. Juanda No.95, Ciputat, Ciputat Timur, South Tangerang 15412, Banten, Indonesia)
DEVITA TETRIANA (Research Center for Radiation Process Technology, National Research and Innovation Agency. Jl. Lebak Bulus Raya No. 49, Cilandak 12440, Jakarta, Indonesia)



Article Info

Publish Date
04 Nov 2023

Abstract

Abstract. Sugoro I, Fadila DSR, Pikoli MR, Hermanto FE, Ramadhan F, Shalsabilla SE, Cici A, Rijal MS, Rahayu DS, Puspito MJ, Haribowo DR, Tetriana D. 2023. Amplicon-based sequencing revealed potential microbiologically influenced corrosion in the interim storage for spent fuel of RSG-GAS, Serpong, Indonesia. Biodiversitas 24: 5391-5398. Interim storage of spent fuel (ISSF) corrosion can negatively impact nuclear power plant maintenance, specifically concerning safety, financial costs, and regulatory compliance. Besides chemical reactions, microbiologically influenced corrosion (MIC) also primarily occurred. Sulfate-reducing bacteria (SRB) plays a significant role in the development of MIC. The quantitative of MIC research has never been conducted in the ISSF - Multipurpose nuclear reactor of G.A. Siwabessy (RSG-GAS). So, this study aims to monitor the potential MIC development in the ISSF of RSG-GAS pool and canal through bacterial diversity analysis using total plate count (TPC) and metagenomic approach. TPC results showed that SRB was identified in the canal of ISSF but not in the pool site. However, metagenomics analysis revealed higher diversity measures in the pool than in the canal sites. Staphylococcus sp. was the most abundant species in both sites, with a relative abundance greater than 85%. The number of SRB and MIC-related bacteria was higher in the canal than in the pool, both from TPC and metagenomic analysis. Several SRB taxa were identified, such as Desulfobulbus mediterraneus, Desulfobulbus sp., Desulfofustis sp., Desulfovibrio sp., and Truepera sp. The potential MIC development also strengthens by diversity-based metabolic pathway prediction, which mainly displayed sulfur oxidation and sulfate reduction pathways in the canal system.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...