Biodiversitas Journal of Biological Diversity
Vol. 24 No. 9 (2023)

Isolation of bacteria displaying potent antagonistic activity against fungi causes anthracnose disease in chili

TRAN THI NGOC TRAM (Institute of Biotechnology, Hue University. Hue 49000, Vietnam)
HOANG TAN QUANG (Institute of Biotechnology, Hue University. Hue 49000, Vietnam)
NGUYEN QUANG HOANG VU (Institute of Biotechnology, Hue University. Hue 49000, Vietnam)
PHAN THI THAO NGUYEN (Institute of Biotechnology, Hue University. Hue 49000, Vietnam)
TON NU MINH THI (QueenLabs Biotechnology One Member Company. Hue 49000, Vietnam)
TRUONG THI BICH PHUONG (University of Sciences, Hue University. Hue 49000 Vietnam)
PHAM THI DIEM THI (Institute of Biotechnology, Hue University. Hue 49000, Vietnam)



Article Info

Publish Date
02 Oct 2023

Abstract

Abstract. Tram TTN, Quang HT, Vu NQH, Nguyen PTT, Thi TNM, Phuong TTB, Thi PTD. 2023. Isolation of bacteria displaying potent antagonistic activity against fungi causes anthracnose disease in chili. Biodiversitas 24: 4919-4926. Anthracnose is a common disease on many crops caused by Colletotrichum species, and it leaves serious consequences, especially on chili. Beta-1,3 glucanase is a semi-constitutive hydrolytic enzyme that is involved in the biological regulation of plant pathogens. Its main function is the breakdown of ?-1,3-glucan, a major constituent of the fungal cell wall. In a study conducted in Vietnam's Quang Tri and Thua Thien Hue Provinces, researchers isolated five bacterial strains from soil where chili plants were grown. To induce the production of beta-1,3 glucanase, these isolates were cultivated in a nutrient-rich medium supplemented with 1% laminarin. The activity of beta-1,3-glucanase in the bacterial strains ranged from 0.31 to 1.72 U mL-1, and antagonistic activity against Colletotrichum spp. causes of anthracnose reached from 50.68 to 69.04%. Strain AT4 had strong antagonistic activity against C. scovillei HUCL1, C. siamensis PV6, and CL3 of 76.44, 62.65, and 68.04%, respectively. Molecular identification based on 16S-rRNA sequences showed that the strains were Paenibacillus polymyxa AT4, B. siamensis ML3, B. tequilensis ML4 and ML6, and B. velezensis GL7. AT4 and ML6 are potential strains for the control of anthracnose diseases caused by Colletotrichum spp. on chili peppers.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...