Biodiversitas Journal of Biological Diversity
Vol. 24 No. 10 (2023)

Diversity of endophytic bacteria in different tissues of sengon (Falcataria moluccana)

MUSTIKA DEWI (School of Life Sciences and Technology, Institut Teknologi Bandung. Jl. Ganesha 10, Bandung 40132, West Java, Indonesia)
NOOR RAHMAWATI (School of Life Sciences and Technology, Institut Teknologi Bandung. Jl. Ganesha 10, Bandung 40132, West Java, Indonesia)
ALFI RUMIDATUL (School of Life Sciences and Technology, Institut Teknologi Bandung. Jl. Ganesha 10, Bandung 40132, West Java, Indonesia)



Article Info

Publish Date
09 Nov 2023

Abstract

Abstract. Dewi M, Rahmawati N, Rumidatul A. 2023. Diversity of endophytic bacteria in different tissues of sengon (Falcataria moluccana). Biodiversitas 24: 5747-5756. Endophytes are endosymbionts, usually bacteria or fungi, that live inside plants without causing disease. Endophytes are available in all plant species and play a role in enhancing plant resistance to insects, pathogens, and herbivores, increasing host growth, nutrient uptake, and increasing plant tolerance to abiotic stresses such as drought. The endophyte's ability to do all that is a result of the production of biological components or secondary metabolites, which may be the result of co-evolution or genetic transfer between endophytic microorganisms and host plants. In this study, the isolation, characterization and identification of endophytic bacteria from several different plant organs of sengon (Falcataria moluccana (Miq.) Barneby & J.W. Grimes) was carried out using the plant piece method in nutrient agar medium, and identification of selected isolates was carried out based on 16S rRNA. 16S rRNA sequencing results showed that 31 endophytic bacterial communities were successfully isolated from various parts of the sengon tree. Bacteria isolated from the stem were 3 strains, bark 7 strains, leaves 11 strains, and galls 10 strains. Molecular identification and phylogenetic analysis showed that the endophytes on the stem were Curtobacterium citreum and C. luteum, respectively. The endophytic bacteria on the bark are C. herbarum, C. luteum, C. pusillum, C. citreum and Azotobacter chroococcum. The endophytic bacteria on the leaves are A. chroococcum, C. citreum, C. herbarum and Lysinibacillus sphaericus. The endophytic bacteria in the gall are Enterobacter ludwigii, Kosakonia radicincitans, Citrobacter gizzii and Erwinia endophytica.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...