Biodiversitas Journal of Biological Diversity
Vol. 25 No. 5 (2024)

Phytocomponents analysis and antioxidant activity of Malacca fruit extract (Phyllanthus emblica) using three different solvents

NAZARUDDIN NAZARUDDIN (Graduate School of Mathematics and Applied Sciences, Universitas Syiah Kuala. Jl. Teuku Nyak Arief No. 441, Banda Aceh 23111, Aceh, Indonesia)
ROSMAIDAR ROSMAIDAR (Laboratory of Pharmacology, Faculty of Veterinary Medicine, Universitas Syiah Kuala. Jl. Teungku Hasan Krueng Kalee No. 4, Banda Aceh 23111, Aceh, Indonesia)
TONGKU N. SIREGAR (Laboratory of Reproduction, Faculty of Veterinary Medicine, Universitas Syiah Kuala. Jl. Teungku Hasan Krueng Kalee No. 4, Banda Aceh 23111, Aceh, Indonesia)
SRI WAHYUNI (Laboratory of Anatomy, Faculty of Veterinary Medicine, Universitas Syiah Kuala. Jl. Teungku Hasan Krueng Kalee No. 4, Banda Aceh 23111, Aceh, Indonesia)
AMALIA SUTRIANA (Laboratory of Pharmacology, Faculty of Veterinary Medicine, Universitas Syiah Kuala. Jl. Teungku Hasan Krueng Kalee No. 4, Banda Aceh 23111, Aceh, Indonesia)



Article Info

Publish Date
12 May 2024

Abstract

Abstract. Nazaruddin, Rosmaidar, Siregar TN, Wahyuni S, Sutriana A. 2024. Phytocomponents analysis and antioxidant activity of Malacca fruit extract (Phyllanthus emblica) using three different solvents. Biodiversitas 25: 1911-1919. Malacca plant (Phyllanthus emblica L.) grows widely in Aceh Province, Indonesia representing a wide group of phytochemical components of medicinal usage. This study aims to assess the antioxidant activity and bioactive compounds in P. emblica fruit extract, which was extracted by subsequent extraction using n-hexane, ethyl acetate, and ethanol. The extracts were then subjected to phytochemical and GC-MS to identify and quantify the bioactive compounds and measure the radical scavenging using DPPH assay to estimate the antioxidant activity. The results showed that P. emblica fruit extracted with ethanol and ethyl acetate contained flavonoids, phenolics, terpenoids, and saponin compounds, while n-hexane extract only contained steroids. The antioxidant activity of ethanol, ethyl acetate, and n-hexane extracts was very strong, with IC50 values of 18.81, 20.75, and 31.01 ppm, respectively. The highest antioxidant activity was found in the ethanol extract of P. emblica fruit. The GC-MS analysis revealed that the most abundant chemical component in ethanol and ethyl acetate extract was 1, 2, 3 benzenetriol, while in n-hexane extract was eugenol. Based on bioactive components and antioxidant activity, ethanol is suggested as the optimal solvent to gain a high content of polyphenols and flavonoids as well as high antioxidants from P. emblica fruit for utilization in pharmacognosy.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...