Yeasts isolated from bovine rumen selected to degrade lignocellulosic roughage

EDUARDO ROBSON DUARTE (Instituto de Ciências Agrárias, Universidade Federal de Minas Gerais. Campus Regional de Montes Claros, Avenida Universitária, 1.000 - Bairro Universitário, Montes Claros-MG-CEP 39.404-547, Brazil)
SABRINA MIRANDA LIMA (Instituto de Ciências Agrárias, Universidade Federal de Minas Gerais. Campus Regional de Montes Claros, Avenida Universitária, 1.000 - Bairro Universitário, Montes Claros-MG-CEP 39.404-547, Brazil)
JULIANE COSTA NERE (Instituto de Ciências Agrárias, Universidade Federal de Minas Gerais. Campus Regional de Montes Claros, Avenida Universitária, 1.000 - Bairro Universitário, Montes Claros-MG-CEP 39.404-547, Brazil)
CLAUDIO EDUARDO SILVA FREITAS (Instituto de Ciências Agrárias, Universidade Federal de Minas Gerais. Campus Regional de Montes Claros, Avenida Universitária, 1.000 - Bairro Universitário, Montes Claros-MG-CEP 39.404-547, Brazil)
HIGOR ALEXANDRE RODRIGUES MAIA (Instituto Federal do Norte de Minas Gerais. Rua Professor Monteiro Fonseca, 216 - Vila Brasília-Montes Claros/MG - CEP 39400-149, Brazil)
ALINE DANIELA LOPES JÚLIO (Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais. Avenida Presidente Antônio Carlos, 6.627, Campus UFMG, Pampulha - Belo Horizonte, MG-CEP 31270-901- Mapa-Contatos, Brazil)
FLÁVIA OLIVEIRA ABRÃO (Instituto Federal Goiano, Campus Ceres. Ceres Goias, Brazil, GO-154, Km 218-Zona Rural, Ceres-GO, CEP76300-000, Brazil)
JANETE MARIA SILVA ALVES (Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais,Brazil, Av. Prof. Rui Braga, s/n - Vila Mauriceia, Montes Claros - MG, 39401-089, Brazil)
VERA LÚCIA DOS SANTOS (Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais. Avenida Presidente Antônio Carlos, 6.627, Campus UFMG, Pampulha - Belo Horizonte, MG-CEP 31270-901- Mapa-Contatos, Brazil)
LUCIANA CASTRO GERASEEV (Instituto de Ciências Agrárias, Universidade Federal de Minas Gerais. Campus Regional de Montes Claros, Avenida Universitária, 1.000 - Bairro Universitário, Montes Claros-MG-CEP 39.404-547, Brazil)
JÚNIO COTA (Instituto de Ciências Agrárias, Universidade Federal de Minas Gerais. Campus Regional de Montes Claros, Avenida Universitária, 1.000 - Bairro Universitário, Montes Claros-MG-CEP 39.404-547, Brazil)

Biodiversitas Journal of Biological Diversity
Vol. 25 No. 3 (2024)

Abstract. Duarte ER, Lima SM, Nere JC, Freitas CES, Maia HAR, Júlio ADL, Abrão FO, Alves JMS, Santos VLD, Geraseev LC, Cota J. 2024. Yeasts isolated from bovine rumen selected to degrade lignocellulosic roughage. Biodiversitas 25: 1159-1166. Commercial yeast strains are viable alternatives to chemical additives in ruminants; however, they are not self to the rumen ecosystem. The present study was conducted to compare the degradation of Urochloa decumbens (Stapf) R.D.Webster (UD) hay and sugarcane bagasse (SB) by eight selected yeast strains, naturally present in the rumen cows and control without microorganisms. Eight yeast isolates that showed >6 log CFU per mL of ruminal fluid were selected and identified as four Pichia kudriavzevii isolates, three Rhodotorula mucilaginosa isolates and one Candida tropicalis isolate, according to their morphological and physiological characteristics and by analyses of the D1 - D2 variable domain sequences of the 28S rRNA gene. The strain V5 (R. mucilaginosa) showed higher cell biomass production than other strains of this species in fermentations with UD (P<0.05). Two isolates of Pichia kudriavzevii (V10 and V62) and one of R. mucilaginosa (V5) promoted a higher reduction of dry matter (DM) of UD than other yeast. The strain V5 was also the most efficient to reduce DM of SB (P <0.05). These selected yeasts could represent a potential alternative for use in animal diets and for industrial applications.