Detection of Legionella pneumophila bacteria from water sources in Palembang City, Indonesia

RADINAL KAUTSAR (Dengue Laboratory, Institute of Tropical Disease, Universitas Airlangga. Jl. Mulyorejo, Kampus C Unair, Surabaya 60115, East Java, Indonesia)
SHIFA FAUZIYAH (Akademi Analisis Kesehatan Delima Husada. Jl. Arif Rahman Hakim No. 2B, Gresik 61111, East Java, Indonesia)
FEBRIANA AQUARESTA (Balai Besar Laboratorium Kesehatan Palembang. Jl. Inspektur Yazid No. 2, Palembang 30126, South Sumatra, Indonesia)
ALICIA MARGARETTA WIDYA (Department of Clinical Microbiology, Faculty of Medicine, Universitas Airlangga. Jl. Prof. Dr. Moestopo 47, Kampus A Unair, Surabaya 60132, East Java, Indonesia)
NUR SAMSYATUL FAJAR (Tropical Disease Diagnostic Center, Institute of Tropical Disase, Universitas Airlangga. Jl. Mulyorejo, Kampus C Unair, Surabaya 60115, East Java, Indonesia)
MAMIK DAMAYANTI (University-CoE Research Center for Bio-Molecule Engineering, Universitas Airlangga. Jl. Mulyorejo, Kampus C Unair, Surabaya 60115, East Java, Indonesia)
TEGUH HARI SUCIPTO (Dengue Study Group, Institute of Tropical Disease, Universitas Airlangga. Jl. Mulyorejo, Kampus C Unair, Surabaya 60115, East Java, Indonesia)

Biodiversitas Journal of Biological Diversity
Vol. 25 No. 4 (2024)

Abstract. Kautsar R, Fauziyah S, Aquaresta F, Widya AM, Fajar NS, Damayanti M, Sucipto TH. 2024. Detection of Legionella pneumophila bacteria from water sources in Palembang City, Indonesia. Biodiversitas 25: 1499-1504. Legionella is a pathogenic bacterial genus that causes legionellosis. This bacteria is a gram-negative bacteria which is often found in warm and humid aquatic environments. Legionella spp. infection occurs due to inhalation of aerosols contaminated with Legionella spp. Until now, legionellosis is still a crucial public health problem in the world. Therefore, it is necessary to take preventive measures to detect the presence of Legionella spp. as early as possible. This study aimed to detect the presence of Legionella pneumophila from well water samples and drinking water sources in Palembang City using the Nested PCR method. The primers used in this study are specific primers that amplify the mip gene. The PCR amplification results showed that 2 of the 22 samples were positively contaminated with L. pneumophila. These positive samples came from well water and drinking water samples. Based on physical parameters, all samples are still safe for consumption because they have a normal temperature, are odorless and clear. Meanwhile, based on chemical parameters, the pH of all samples is still safe for consumption because it is still below the maximum threshold.