Antibiotic production by an endophytic Streptomyces isolated from the medicinal plant Poikilospermum suaveolens

ALIMUDDIN ALI (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Makassar. Jl. Daeng Tata, Makassar 90222, South Sulawesi, Indonesia)
MUHARRAM PASSITTA (Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Makassar. Jl. Daeng Tata, Makassar 90222, South Sulawesi, Indonesia)
HERLINA RANTE (Faculty of Pharmacy, Universitas Hasanuddin. Jl. Perintis Kemerdekaan KM 10, Makassar 90245, South Sulawesi, Indonesia)
ELLY WAHYUDIN (Faculty of Pharmacy, Universitas Hasanuddin. Jl. Perintis Kemerdekaan KM 10, Makassar 90245, South Sulawesi, Indonesia)
NANA JUNIARTI NATSIR DJIDE (Faculty of Pharmacy, Universitas Hasanuddin. Jl. Perintis Kemerdekaan KM 10, Makassar 90245, South Sulawesi, Indonesia)
ROLAND JANSEN POLITAN (Department of Microbial Chemistry, Graduate School of Pharmaceutical Sciences, Kitasato University. Tokyo 108-8641, Japan)
ELYZA AIMAN NUR (Department of Microbial Chemistry, Graduate School of Pharmaceutical Sciences, Kitasato University. Tokyo 108-8641, Japan)
SATORU SHIGENO (Department of Microbial Chemistry, Graduate School of Pharmaceutical Sciences, Kitasato University. Tokyo 108-8641, Japan)
SATOSHI OHTE (Department of Microbial Chemistry, Graduate School of Pharmaceutical Sciences, Kitasato University. Tokyo 108-8641, Japan)
KEISUKE KOBAYASHI (Department of Microbial Chemistry, Graduate School of Pharmaceutical Sciences, Kitasato University. Tokyo 108-8641, Japan)
KANJI HOSODA (Laboratory of Drug Discovery, Graduate School of Pharmaceutical Sciences, Kitasato University. Tokyo 108-8641, Japan)
HIROSHI TOMODA (Laboratory of Drug Discovery, Graduate School of Pharmaceutical Sciences, Kitasato University. Tokyo 108-8641, Japan)
TAICHI OHSHIRO (Department of Microbial Chemistry, Graduate School of Pharmaceutical Sciences, Kitasato University. Tokyo 108-8641, Japan)

Biodiversitas Journal of Biological Diversity
Vol. 25 No. 5 (2024)

Abstract. Ali A, Passitta M, Rante H, Wahyudin E, Djide NJN, Politan RJ, Nur EA, Shigeno S, Ohte S, Kobayashi K, Hosoda K, Tomoda H, Ohshiro T. 2024. Antibiotic production by an endophytic Streptomyces isolated from the medicinal plant Poikilospermum suaveolens. Biodiversitas 25: 2221-2229. This study investigates the antibiotic production potential of endophytic actinomycetes isolated from Poikilospermum suaveolens plants. Samples were collected from various parts of the plant in karst ecosystems. Endophytic actinomycetes were cultured and screened for antimicrobial activity using agar diffusion and liquid microdilution methods. One of the 11 isolated strains exhibited significant antibiotic activity against clinically relevant organisms identified as belonging to the Streptomyces genus, specifically Streptomyces mirabilis strain NRRL ISP-5169. Submerged fermentation of this strain over 9 days showed strong antagonistic effects against Gram-positive bacteria. Spectroscopic analyses confirmed the presence of actinomycin D as the bioactive compound. This study provides on the previously unexplored potential of endophytic actinomycetes from P. suaveolens as a promising resource for antibiotic discovery.