Biodiversitas Journal of Biological Diversity
Vol. 25 No. 10 (2024)

Exploration of cadmium-resistant bacteria in cadmium-contaminated rice fields in Karawang District, Indonesia

NOVRIANTY RIZQI AZIS (Graduate School of Soil and Water Management, Faculty of Agriculture, Universitas Brawijaya. Jl. Veteran No. 10-11, Malang 65145, East Java, Indonesia)
YULIA NURAINI (Department of Land Resource Management, Faculty of Agriculture, Universitas Brawijaya. Jl. Veteran, No. 10-11, Malang 65145, East Java, Indonesia)
CAHYO PRAYOGO (Department of Land Resource Management, Faculty of Agriculture, Universitas Brawijaya. Jl. Veteran, No. 10-11, Malang 65145, East Java, Indonesia)
RIZKY MAULANA ISHAQ (Department of Land Resource Management, Faculty of Agriculture, Universitas Brawijaya. Jl. Veteran, No. 10-11, Malang 65145, East Java, Indonesia)



Article Info

Publish Date
31 Oct 2024

Abstract

Abstract. Azis NR, Nuraini Y, Prayogo C, Ishaq RM. 2024. Exploration of cadmium-resistant bacteria in cadmium-contaminated rice fields in Karawang District, Indonesia. Biodiversitas 25: 3637-3644. Cadmium is a heavy metal naturally present in the soil, but its presence can increase due to human activities (anthropogenic). Increased cadmium content in soil due to human activities can occur due to agricultural activities, such as the continuous use of chemical fertilizers and pesticides. These chemical products contain harmful heavy metals such as cadmium, which results in soil pollution due to the accumulation of these metals in the soil and the entry of these metals into agricultural products, thus affecting health. The problem can be solved by soil bioremediation that utilizes indigenous bacteria. This study aims to explore cadmium-resistant bacteria with high resistance levels that can be used for the bioremediation of cadmium-contaminated soil. Cadmium-resistant bacteria were explored from 4 locations in Karawang District, Indonesia, with soil cadmium content >0.5 ppm, namely 2 locations in Telukjambe Village, 1 in Purwajaya Village, and 1 in Lemahmukti. 14 cadmium-resistant bacterial isolates with different characteristics were obtained from these 4 locations. Microbiological tests conducted include Gram staining test, resistance test, pathogenicity test, and bacterial DNA test. Seven bacterial isolates were found to be non-pathogenic to humans and plants. The best three bacterial isolates from 7 non-pathogenic isolates with resistance ability 50-500 ppm can potentially be used as a bioremediation of cadmium-contaminated soil. The three isolates are isolates B1, B6, and B12. DNA test results stated that isolate B1 is Shewanella decolorationis strain NSSD01, B6 is Chryseobacterium cucumeris strain E1-3, and B12 is Aeromonas hydrophila strain GX5.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...