Genetic polymorphism of Populus pruinosa and Populus euphratica in Kazakhstan based on ISSR markers

DINARA AKHATAYEVA (Department of Biology and Biotechnology, Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University. 71 Al-Farabi Ave., Almaty 050040, Kazakhstan)
MAKSIM KUTSEV (Altai State University. 61 Lenina Ave., Barnaul 656049, Russia)
POLINA VESSELOVA (Institute of Botany and Phytointroduction. Timiryazev Str. 36D/1, Almaty 050040, Kazakhstan)
ZARINA INELOVA (Department of Biology and Biotechnology, Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University. 71 Al-Farabi Ave., Almaty 050040, Kazakhstan)
BEKTEMIR OSMONALI (Institute of Botany and Phytointroduction. Timiryazev Str. 36D/1, Almaty 050040, Kazakhstan)
MENGTAY AITZHAN (Department of Fundamental Medicine, Faculty of Medicine and Health, Al-Farabi Kazakh National University. 71 Al-Farabi Ave., Almaty 050040, Kazakhstan)
YELENA ZAPARINA (Department of Biology and Biotechnology, Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University. 71 Al-Farabi Ave., Almaty 050040, Kazakhstan)
LAURA SHADMANOVA (Institute of Botany and Phytointroduction. Timiryazev Str. 36D/1, Almaty 050040, Kazakhstan)
EMIL BOROS (Institute of Aquatic Ecology, HUN-REN Centre for Ecological Research. Karolina St. 29, Budapest H-1113, Hungary)

Biodiversitas Journal of Biological Diversity
Vol. 26 No. 12 (2025)

Abstract. Akhatayeva D, Kutsev M, Vesselova P, Inelova Z, Osmonali B, Aitzhan M, Zaparina Y, Shadmanova L, Boros E. 2025. Genetic polymorphism of Populus pruinosa and Populus euphratica in Kazakhstan based on ISSR markers. Biodiversitas 26: 6120-6129. The genus Populus (Salicaceae) in Kazakhstan is represented by 13 species, although the exact number of species, including varieties and hybrids, has not been clearly established. Among them, Populus pruinosa and Populus euphratica are native species with rapidly declining population sizes and shrinking habitats. Comprehensive studies on their biology and genetic diversity in Kazakhstan have not reported so far. This study aimed to assess genetic diversity, compare patterns of differentiation, evaluate the relationship between genetic variation and ecological conditions in natural populations of both species. Genetic variability was assessed using the ISSR method, and statistical analysis was performed using ANOVA. Samples from six natural populations in southern Kazakhstan (three populations of P. pruinosa and three of P. euphratica) showed distinct interspecific differentiation and variable genetic diversity. P. pruinosa populations had higher allelic diversity (h = 0.114-0.153) and expected heterozygosity (0.107-0.139) than P. euphratica (h = 0.041-0.085). Greater genetic diversity in P. pruinosa indicates stronger adaptive potential to arid environments. A moderate correlation was found between genetic and geographic distances (r = 0.597). The findings highlight P. pruinosa superior ecological adaptability. Both species are recommended for targeted conservation and restoration of arid ecosystems in Kazakhstan.