Biodiversitas Journal of Biological Diversity
Vol. 27 No. 5 (2026)

Morphological traits and non-destructive leaf area estimation of Moringa oleifera under different stem cutting lengths

FITRA GUSTIAR (Faculty of Agriculture, Universitas Sriwijaya. Jl. Raya Palembang-Prabumulih Km. 32, Ogan Ilir 30662, South Sumatra, Indonesia)
ROFIQOH PURNAMA RIA (Faculty of Agriculture, Universitas Sriwijaya. Jl. Raya Palembang-Prabumulih Km. 32, Ogan Ilir 30662, South Sumatra, Indonesia)
NABILA AYU KRHISMA (Faculty of Agriculture, Universitas Sriwijaya. Jl. Raya Palembang-Prabumulih Km. 32, Ogan Ilir 30662, South Sumatra, Indonesia)



Article Info

Publish Date
25 Jun 2026

Abstract

Abstract. Gustiar F, Ria RP, Krhisma NA. 2026. Morphological traits and non-destructive leaf area estimation of Moringa oleifera under different stem cutting lengths. Biodiversitas 27 (5): d270523. https://doi.org/10.13057/biodiv/d270523. Moringa oleifera is widely cultivated for its highly nutritious leaves, making efficient propagation and accurate growth evaluation essential for improving leaf productivity. This study aimed to evaluate the effects of different stem cutting lengths on the morphological traits and early growth of M. oleifera and to develop a non-destructive method for estimating leaf area. The experiment was conducted in a greenhouse using a randomized complete block design with five stem cutting lengths (20, 30, 40, 50, and 60 cm). Leaf area estimation models were developed using measurements of leaf length and width of fully expanded compound leaves using regression analysis. Among the evaluated models, the linear regression based on leaf Length × Width (L × W) produced the highest predictive performance (R² = 0.9636) within the calibration dataset used in this study. Stem cutting length was associated with variation in early growth responses. Cuttings of approximately 40 cm generally showed more favorable early-growth characteristics, including higher survival percentage, larger leaf area, and greater dry biomass accumulation compared with several other treatments. Because survival data were derived from a limited number of plants per treatment, survival responses are interpreted descriptively. Differences in fresh biomass variables were not statistically significant, although variation was observed in leaf and root dry weight. These findings suggest that moderate cutting length may support balanced early establishment of M. oleifera under greenhouse conditions. The developed leaf area model provides a practical tool for repeated growth monitoring without destructive sampling. However, because the model was not validated using an independent dataset, further testing under field conditions and across broader environmental settings is still required before wider application.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...