Jejak digital: Jurnal Ilmiah Multidisiplin
Vol. 2 No. 5 (2026): AGUSTUS-SEPTEMBER

Determination of Caffeine Content in Green Coffea liberica Beans by UV–Visible Spectrophotometry and Evaluation of Its Effect on Weight Reduction

Danik Sulistyoningsih (University Telogorejo Semarang, Indonesia)
Firstca Aulia Rachma (University Telogorejo Semarang, Indonesia)
Silvy Aldila (University Telogorejo Semarang, Indonesia)
Suksi Riani (University Telogorejo Semarang, Indonesia)
Winnie Kristiyanti (University Telogorejo Semarang, Indonesia)
Recta Syifa Dwimart (University Telogorejo Semarang, Indonesia)



Article Info

Publish Date
04 Aug 2026

Abstract

Background: Green coffee beans (Coffea liberica) are a rich source of bioactive compounds, including caffeine, chlorogenic acid, flavonoids, and polyphenols, which have potential health-promoting properties. Caffeine has been reported to enhance thermogenesis, stimulate fat oxidation, and increase energy expenditure, making it a promising natural compound for weight management. This study aimed to determine the caffeine content of green Coffea liberica beans using UV–Visible spectrophotometry and to evaluate the implementation of green coffee extract consumption on body weight reduction. Methods: An experimental study was conducted by extracting green coffee beans with 96% ethanol followed by phytochemical screening and quantitative determination of caffeine using UV–Visible spectrophotometry at a maximum wavelength of 272 nm. The analytical method was validated by assessing linearity, accuracy, and precision. A one-group pretest–posttest design was employed to evaluate the implementation of green coffee extract in overweight adults with a body mass index (BMI) ≥25 kg/m². Body weight was measured before and after the intervention, and statistical analysis was performed at a 95% confidence level. Results: The spectrophotometric method demonstrated satisfactory linearity, accuracy, and precision, indicating its suitability for caffeine determination. Green Coffea liberica beans were confirmed to contain caffeine and other bioactive compounds that contribute to antioxidant activity. Consumption of green coffee extract showed a tendency to reduce body weight following the intervention period. Conclusion: UV–Visible spectrophotometry is a reliable method for determining caffeine content in green Coffea liberica beans. The presence of caffeine and other bioactive compounds supports the potential application of green Liberica coffee as a functional food ingredient for weight management.

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Journal Info

Abbrev

jejakdigital

Publisher

Subject

Economics, Econometrics & Finance Education Languange, Linguistic, Communication & Media Social Sciences Other

Description

Jurnal Ilmiah Multidisiplin adalah jurnal elektronik dan cetak Open Access Journal yang diterbitkan oleh Indo Publishing setiap 6 kali dalam setahun menyediakan forum untuk mempublikasikan artikel penelitian asli, artikel review dari kontributor, dan berita teknologi baru mencangkup multidisiplin ...