Nusantara Bioscience
Vol. 8 No. 1 (2016)

The effects of physical and hormonal treatments on dormancy breaking and the changes in seed coat ultrastructure of Delonix regia

SOLICHATUN SOLICHATUN (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret University. Jl. Ir. Sutami 36A Surakarta 57126, Central Java, Indonesia)
SANTOSA SANTOSA (Faculty of Biology, Universitas Gadjah Mada. Sleman 55281, Yogyakarta, Indonesia)
KUMALA DEWI (Faculty of Biology, Universitas Gadjah Mada. Sleman 55281, Yogyakarta, Indonesia)
RARASTOETI PRATIWI (Faculty of Biology, Universitas Gadjah Mada. Sleman 55281, Yogyakarta, Indonesia)



Article Info

Publish Date
01 Jan 2016

Abstract

Abstract. Solichatun, Santosa, Dewi K, Pratiwi R. 2016. The effects of physical and hormonal treatments on dormancy breaking and the changes in seed coat ultrastructure of Delonix regia. Nusantara Bioscience 8: 94-102. The seed dormancy is described as the inability of an intact viable seed to complete germination under favorable conditions. Delonix regia (Hook) Raf. also known as flamboyant is a member of Fabaceae that has seed dormancy. Every seed has a different structure in their seed coat that responsible for initiating imbibition of water due to dormancy breakdown and germination. The aims of this research were to investigate the effect of physical and hormonal treatment in dormancy break of flamboyant seed and to investigate the structure of seed coat on the dormancy and germination. The treatments were applied to break the dormancy of D. regia seeds were physical treatments and hormonal treatments (ABA, BAP, GA, and IBA). Morphological and anatomical changes during dormancy breakdown were investigated using scanning electron microscope. The imbibition pathway tracked using blocking experiment. The results indicated that physical treatment that seeds dipped in hot water (98oC) for 5 minutes was the most effective treatment to dormancy breakdown of flamboyant seeds (95%). The application of GA3 100 ppm results in the highest of germination percentage (22%) among other hormones. The result of blocking experiment showed that hilum and lens play an important role during imbibition processes; and the type of flamboyant seed water gap was the lens gap type.

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Aims and Scope Nusantara Bioscience (Nusantara Biosci) encourages submission of manuscripts dealing with all aspects of biological sciences that emphasize issues germane to biological and nature ...