Nusantara Bioscience
Vol. 9 No. 2 (2017)

Dental caries bacterial isolates sensitivity toward water-, ethanolicextract and essential oils of several shallot cultivars (Allium cepa)

IDA INDRAWATI (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran. Jl. Raya Bandung-Sumedang Km 21 Jatinangor, Sumedang 45363, West Java)
AULIA PONNY ANGGRAINI (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran. Jl. Raya Bandung-Sumedang Km 21 Jatinangor, Sumedang 45363, West Java)
BUDI IRAWAN (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran. Jl. Raya Bandung-Sumedang Km 21 Jatinangor, Sumedang 45363, West Java)



Article Info

Publish Date
20 May 2017

Abstract

Indrawati I, Anggraini AP, Irawan B. 2017. Dental caries bacterial isolates sensitivity toward water-, ethanolic- extract and essential oils of several shallot cultivars (Allium cepa). Nusantara Bioscience 9: 229-235. Sensitivity test of dental caries bacterial isolates has been carried out toward water-, ethanolic- extract and essential oil of Batu, Maja, Sumenep shallot cultivars. The experimental method was conducted using sensitivity test through Kirby-Bauer method with randomized complete design factorial 5 x 16 for each shallot cultivars with two replications. Factor one (i) was dental caries bacteria isolates (Lactobacillus sp., Staphylococcus sp.1, Staphylococcus sp.2, Staphylococcus sp.3 and Streptococcus sp.). The other factor (ii) was an antibacterial agent (water-extract, ethanolic- extract and essential oil of Batu, Maja, Sumenep shallot cultivars and antibiotic Vancomycine 30 μg). Concentration for each shallot cultivars extract that used was 80%, 40%, 20%, 10% and 0%. The result of sensitivity test to those antibacterial agents showed that 80% water extract of Maja cultivar had the best inhibition zone of Staphylococcus sp.1 growth with the average of inhibition zone 37 mm. The interaction factor between essential oil from Batu and Maja as well as Sumenep shallot cultivar revealed a better inhibition zone than Vancomycine 30 μg with all bacterial isolates tested.

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Aims and Scope Nusantara Bioscience (Nusantara Biosci) encourages submission of manuscripts dealing with all aspects of biological sciences that emphasize issues germane to biological and nature ...