The phagocytosis activity of isoeugenol-ester compound on Mus musculus macrophage cell

AWIK PUJI DYAH NURHAYATI (Department of Biology, Faculty of Science, Institut Teknologi Sepuluh Nopember. Jl. Arief Rahman Hakim, Surabaya 60111, East Java, Indonesia)
MARDI SANTOSO (Department of Chemistry, Faculty of Science, Institut Teknologi Sepuluh Nopember. Jl. Arief Rahman Hakim, Surabaya 60111, East Java, Indonesia)
EDWIN SETIAWAN (Department of Biology, Faculty of Science, Institut Teknologi Sepuluh Nopember. Jl. Arief Rahman Hakim, Surabaya 60111, East Java, Indonesia)
DEAVINA THALITA RACHMA (Department of Biology, Faculty of Science, Institut Teknologi Sepuluh Nopember. Jl. Arief Rahman Hakim, Surabaya 60111, East Java, Indonesia)

Nusantara Bioscience
Vol. 11 No. 2 (2019)

Abstract. Authors. 2019. The activity phagocytosis of isoeugenol-ester compound on Mus musculus macrophage cell. Nusantara Bioscience 11: 117-122. Chemotherapy is drugs therapy for cancer. However, chemotherapy could damage normal cells and decrease immune system. For this reason, it is necessary to find immunostimulant compound that is able to increase immune system. Isoeugenol-ester is unnatural compound that has been tested for its cytotoxicity against cancer cell and showed positive result. Nevertheless, ability of Isoeugenol-ester for inducing phagocytosis is still unknown. Therefore, bioassays study using in vitro phagocytosis were performed. Latex beads methods using antigen model in 5 isoeugenol-ester concentration series: 6, 25; 12, 5; 25; 50 and 100 ?g/mL were tested to Mus musculus peritoneal macrophage cell. The optimum concentration of Isoeugenol-ester compounds in inducing phagocytosis activity of M. musculus macrophage cell was 12.5 ?g/mL.