Diversity of arbuscular mycorrhizal fungi and root colonization in Polygonatum verticillatum

AJAY KUMAR (Himalayan Forest Research Institute. Conifer Campus, Shimla, Himachal Pradesh 171013, India.)
ASHWANI TAPWAL (Himalayan Forest Research Institute. Conifer Campus, Shimla, Himachal Pradesh 171013, India.)

Nusantara Bioscience
Vol. 14 No. 1 (2022)

Abstract. Kumar A, Tapwal A. 2022. Diversity of arbuscular mycorrhizal fungi and root colonization in Polygonatum verticillatum. Nusantara Bioscience 14: 53-63. Diversity of arbuscular mycorrhizal fungi (AMF) associated with Polygonatum verticillatum (L.) All. was investigated in two sites of Himachal Himalaya, India. A total of 15 AMF species were isolated and identified from the rhizosphere soil of P. verticillatum. The spore density was 1.48 ± 1.91 and 3.99 ± 3.78 per 20 grams of rhizosphere soil at the site I and site II, respectively. Mycorrhizal colonization in the roots of P. verticillatum was recorded at 46.12 and 52.23 percent at the site I and site II, respectively. In addition, the mycorrhizal structures like darkly stained endophytic hyphae, coiled intracellular hyphae, Y-shaped hyphae, and 'H' connection (Arum type) were recorded.