Influence of media variations and growth regulators on in vitro propagation of Dendrocalamus asper bamboo

WAN NURFARZANA WAN MOHAMAD ZANI (Department of Biology, Faculty of Applied Science, Universiti Teknologi MARA Shah Alam. 40450 Shah Alam, Selangor, Malaysia)
NORRIZAH JAAFAR SIDIK (Department of Biology, Faculty of Applied Science, Universiti Teknologi MARA Shah Alam. 40450 Shah Alam, Selangor, Malaysia)
NUR AFIQAH MOHD ISA (Department of Biology, Faculty of Applied Science, Universiti Teknologi MARA Shah Alam. 40450 Shah Alam, Selangor, Malaysia)
ASMAH AWAL (Agricultural Biotechnology Research Group, Faculty of Plantation and Agrotechnology, Universiti Teknologi MARA Jasin Campus. 77300, Merlimau, Malacca, Malaysia)
NURUL IZZATI OSMAN (Department of Pharmacology and Pharmaceutical Chemistry, Faculty of Pharmacy, Universiti Teknologi MARA Puncak Alam Campus. 42300 Bandar Puncak Alam, Selangor, Malaysia)
NORALIZA ALIAS (Forest Biotechnology Division, Forest Research Institute Malaysia. 52109 Kepong, Selangor, Malaysia)
MOHD KHAIRI NORDIN (School of Electrical Engineering, College of Engineering, Universiti Teknologi MARA Shah Alam. 40450 Shah Alam, Selangor, Malaysia)

Nusantara Bioscience
Vol. 16 No. 2 (2024)

Abstract. Zani WNWM, Sidik NJ, Isa NAM, Awal A, Osman NI, Alias N, Nordin MK. 2024. Influence of media variations and growth regulators on in vitro propagation of Dendrocalamus asper bamboo. Nusantara Bioscience 16: 237-244. This study reports the effects of different types of media and combination of plant growth regulators on the in vitro propagation of Dendrocalamus asper (Schult.f.) Backer bamboo species, a species known for its economic significance and challenges in traditional cultivation. The experiment was initiated through the cultivation of in vitro nodal segments as explants in various strengths (half-strength and full-strength) of Murashige and Skoog’s (MS) and Vacin & Went (VW) media, supplemented with 1.0 mg L-1 6-benzylaminopurine (BAP) hormone. Optimal results were achieved in full-strength MS media with 1.0 mg L-1 BAP, with the highest shoot number (5.6 shoots) and length (2.14±0.18 cm), surpassing outcomes in VW media. Shoot multiplication in full-strength MS media, with varying BAP and indole-3-butyric acid (IBA) hormone combinations (0.5, 1.0, 2.0 and 4.0 mg L-1) was conducted, and the combination of 4.0 mg L-1 BAP and 0.5 mg L-1 IBA yielded the highest number of shoots (5.17±3.97), while supplementation with 4.0 mg L-1 IBA alone resulted in the longest shoot with 1.89±1.15 cm. These findings underscore the significance of tailored conditions for optimal in vitro propagation of this species. Further investigations could explore additional factors influencing the propagation process for better refinement of bamboo cultivation techniques.