Exploration Of The Potential Of Phaleria Macrocarpa Against Colon Cancer Using A Network Pharmacology Approach Network Pharmacology-Based Exploration of Phaleria Against Colon Cancer

Laili Khusnul Khotimah (a:1:{s:5:"en_US"s:37:"Universitas Buana Perjuangan Karawang"})
Restia Prasisila Hidayanti Nawangsasih
Diana Magdalena Larassati
Syalzhabilla Yasmin
Adelia NurFitri
Himyatul Hidayah

International Journal of Health, Engineering and Technology
Vol. 5 No. 2 (2026): Vol 5. No. 2 JULY 2026

Colon cancer is one of the leading causes of cancer-related mortality worldwide. Phaleria macrocarpa contains various bioactive secondary metabolites with potential anticancer activity. This study aimed to identify the potential molecular targets of Phaleria macrocarpa against colon cancer using a network pharmacology approach. Compound data were collected from LC-MS/MS-based studies and screened using pkCSM. Protein targets were predicted using SuperPred and compared with colon cancer-related proteins obtained from GeneCards. Protein interaction and network topology analyses were performed using STRING-DB, Cytoscape, and CytoNCA. The results identified 10 overlapping target proteins, namely TLR4, GLS, MAP2K2, LDHA, STAT3, NFKB1, PTGS1, GSK3B, STAT1, and SLC2A1. Network analysis revealed that NFKB1 were the key targets with the highest centrality values. These findings suggest that Phaleria macrocarpa may inhibit colon cancer progression through multiple molecular targets and biological pathways.