This study aims to analyze the effect of using Articulate Storyline learning media on students' learning motivation in science subjects. The research employed a quantitative approach with a pre-experimental one-group pretest-posttest design. The sample consisted of 32 students of class VII H at SMP Negeri 19 Semarang. Data were collected through a learning motivation questionnaire administered before and after the treatment. The results showed that the average learning motivation score increased from 71.69 to 85.06. The paired sample t-test yielded a significance value of 0.001 (< 0.05), indicating a significant effect of Articulate Storyline on students' learning motivation. The N-Gain value was 0.5433, categorized as moderate. These findings indicate that Articulate Storyline is effective in increasing students' motivation in science learning. Keywords: Articulate Storyline, learning motivation, science learning, interactive media
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