PENDIPA Journal of Science Education
Vol 10 No 1 (2026): January - March

The Profile of Allelochemical Compounds in Ratun Sorghum Root Extract Based on GC-MS Analysis as a Bioherbicide Candidate: Profil Senyawa Alelokimia Ekstrak Akar Ratun Sorgum Berdasarkan Analisis GC-MS sebagai Kandidat Bioherbisida

Edi Susilo (Program Studi Agroteknologi, University of Ratu Samban)
Nanik Setyowati (Program Studi Agroekoteknologi, Universitas Bengkulu)
Uswatun Nurjanah (Program Studi Agroekoteknologi, Universitas Bengkulu)
Hesti Pujiwati (Program Studi Agroekoteknologi, Universitas Bengkulu)
Riwandi (Program Studi Ilmu Tanah, Universitas Bengkulu)



Article Info

Publish Date
30 Jan 2026

Abstract

From the past until now, weed control using synthetic herbicides has remained the primary method. The use of synthetic herbicides in modern agricultural processes still has negative impacts on the environment, health, and weeds, thereby potentially causing weed resistance. Therefore, planting herbal bioherbicides is a more environmentally friendly method of weed control. Sorghum (Sorghum bicolor L. Moench), in particular, has allelopathic exudates that are very troublesome for weeds. This study focused on analyzing the chemical allelopathy of sorghum root extracts and identifying candidate bioherbicide compounds in the roots using Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Sorghum was extracted using a cold maceration method obtained from a mixture of solvents with different configurations. Then, through GC-MS, the solvents were extracted, and the compounds were separated. As a result, through GC-MS analysis, 20 compounds were identified, most of which were detected in the steroid, sterol, and fatty acid derivative groups. The most prominent compounds detected were 4, 22-stigmastadien-3-one (20.74%); stigmasterol (total 16.47%); cholest-4-en-26-oic acid, 3-oxo- (11.68%); and 9-octadecenamide (Z) (7.69%). Several polar compounds, such as 9-octadecenamide (Z) and cyclohexanol derivatives, exhibited excellent capabilities. Therefore, sorghum root extract contains allelochemical compounds that have the potential to be sustainable and environmentally friendly bioherbicides.

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Journal Info

Abbrev

pendipa

Publisher

Subject

Chemistry Education Mathematics

Description

PENDIPA Journal of Science Education is a peer-reviewed, open-access journal covered all aspect of science and science education. PENDIPA journal welcomes the submission of scientific articles related to mathematics, physics, chemistry, biology, and its educational implementation in a school, higher ...