Utilising PLS-Enhanced FTIR Spectroscopy for the Rapid Screening and Quantification of Chemical Additives in Traditional Jamu: A Case Study on Vitamin B6

Fajar Aji Lumakso (Department of Pharmacy, Faculty of Mathematic and Natural Sciences, Universitas Islam Indonesia, Yogyakarta, Indonesia)
Ardi Nugroho (Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Islam Indonesia)
Sucy Cahyani Putri (Student of Undergraduate Program of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Islam Indonesia)
Chandra Kartika Nawangsari (Student of Master Program of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Islam Indonesia)

Journal of Food and Pharmaceutical Science
Vol 14, No 4 (2026): J.Food.Pharm.Sci (In Press)

Until today, there are still many medicinal chemicals added to jamu to gain the interest of the consumers. This illegal practice can lead to serious side effects for health. This research was intended to develop fast screening to identify and to quantify the vitamin B6 (pyridoxine) in herbal product using FTIR spectroscopy allied with partial least square (PLS). The thin layer chromatography-densitometry was also employed to strangthen the identification. For the quantification, several potential wavenumbers were employed between 4000-400 cm-1. These spectral dataset then was analysed using XLSTAT. The R2 results obtained were 0.9898; RMSEC 0.001170; RMSECV 0.003808 and PRESS 0.000087 showing good results of developed model. The findings revealed the potential of FTIR and PLS combination to be extended as tools for analysing the marketed herbal products mixed with the medicinal chemicals.