The Negeri Larike Eel Pond is a nature-based tourist destination whose main attraction is the presence of eels (Anguilla bicolor), which are an integral part of the local community’s traditional knowledge and whose conservation remains a priority to this day. This study aims to identify the ecotourism potential of the Negeri Larike Eel Pond tourist site based on the area’s biophysical conditions particularly the presence of flora and fauna around the site as well as the components of attractions, accessibility, and amenities. The study employs a survey method with a qualitative descriptive approach. Data were collected through field observations, in-depth interviews, and documentation. Flora and fauna were identified using the iNaturalist app and field guides, and assessed using Fandeli’s (2005) criteria. The results indicate that the Kolam Morea tourist site area possesses potential supporting ecotourism development, with 25 identified flora species falling into the “good” category, including two species of high conservation value, namely Intsia bijuga (NT) and Pterocarpus indicus (EN), while 8 fauna species fall into the “moderate” category, including three protected species: the spotted cuscus, eclectus parrot, and Timor deer. The area’s main attraction is the Morea, classified as Near Threatened (NT-IUCN), weighing approximately 10 kg with a length of 1.5–2 meters, which is protected through the local community’s traditional knowledge and reinforced by a feeding activity that draws visitors. Access to the site is possible by both two-wheeled and four-wheeled vehicles over a distance of 40.1 km (±1.3 hours) from Ambon City, although public transportation remains limited. Basic amenities such as parking areas, restrooms, changing rooms, a prayer room, gazebos, seating areas, and food stalls are available, though some facilities require improvement, particularly waste disposal facilities. This area has the potential to be developed as an ecotourism destination based on conservation and local wisdom.
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